QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Moehn, S. Articles by Ball, R. O. Search for Related Content PubMed PubMed Citation Articles by Moehn, S. Articles by Ball, R. O.

---

Nutrient Metabolism

Indicator Amino Acid Oxidation Responds Rapidly to Changes in Lysine or Protein Intake in Growing and Adult Pigs^1,2

Soenke Moehn^*, Robert F.P. Bertolo^*,3, Paul B. Pencharz^*, and Ronald O. Ball^*,4

^* Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada, T6G 2P5 and Research Institute, Hospital for Sick Children, Toronto, ON, Canada, M5G 1X8

⁴To whom correspondence should be addressed. E-mail: Ron.Ball{at}ualberta.ca.

There is disagreement about the adaptation time required when using the indicator amino acid oxidation (IAAO) technique. Our objective was to establish the adaptation time required to obtain a plateau in indicator (L-[1-¹⁴C]-phenylalanine) oxidation in response to a test diet using growing and adult pigs. Four barrows (20 kg) and 4 sows (240 kg) were surgically implanted with venous catheters for isotope infusion. Growing Pigs: After 7 d of adaptation to an adequate lysine intake of 8.8 g/d, phenylalanine oxidation in growing pigs was 9.38 ± 1.25% of the infused dose. At 2, 3, 4, or 6 d after reducing lysine intake to 3.8 g/d, and then increasing it back to 8.8 g/d, phenylalanine oxidation was 16.94 ± 0.84% (P < 0.05) and 9.70 ± 0.80% (P < 0.05), respectively, with no significant effect of days of adaptation to diet. Adult Pigs: After 14 d of adaptation to an intake of 200% of the amino acid maintenance requirement, phenylalanine oxidation in sows was 4.23 ± 0.45% of dose. Changing the intake to 100 and 50% of the maintenance requirement, increased (P < 0.05) phenylalanine oxidation to 5.95 ± 0.26 and 7.90 ± 0.26%, respectively, with no significant effect of time (1, 2, 5, 6, 9, and 10 d) after diet change. The CV for repeated phenylalanine oxidation measurements within pigs and diets was 13.5% for growing and 8.8% for adult pigs. This demonstrates that the IAAO requires <2 d of adaptation regardless of age, dietary challenge (individual amino acid or total protein) or direction (increase or decrease) of change, and that the measured oxidation rate (% of dose) is highly repeatable.

KEY WORDS: • adaptation • amino acid kinetics • pigs • indicator amino acid oxidation

This article has been cited by other articles:

				R. Elango, R. O. Ball, and P. B. Pencharz Indicator Amino Acid Oxidation: Concept and Application J. Nutr., February 1, 2008; 138(2): 243 - 246. [Abstract] [Full Text] [PDF]

				S. Moehn, A. K. Shoveller, M. Rademacher, and R. O. Ball An estimate of the methionine requirement and its variability in growing pigs using the indicator amino acid oxidation technique J Anim Sci, February 1, 2008; 86(2): 364 - 369. [Abstract] [Full Text] [PDF]

				M. A Humayun, R. Elango, R. O Ball, and P. B Pencharz Reevaluation of the protein requirement in young men with the indicator amino acid oxidation technique Am. J. Clinical Nutrition, October 1, 2007; 86(4): 995 - 1002. [Abstract] [Full Text] [PDF]

				M. A. Humayun, R. Elango, S. Moehn, R. O. Ball, and P. B. Pencharz Application of the Indicator Amino Acid Oxidation Technique for the Determination of Metabolic Availability of Sulfur Amino Acids from Casein versus Soy Protein Isolate in Adult Men J. Nutr., August 1, 2007; 137(8): 1874 - 1879. [Abstract] [Full Text] [PDF]

				F. Ji, W. L. Hurley, and S. W. Kim Characterization of mammary gland development in pregnant gilts J Anim Sci, March 1, 2006; 84(3): 579 - 587. [Abstract] [Full Text] [PDF]

				A. V. Kurpad, M. M. Regan, T. Raj, and J. V. Gnanou Branched-Chain Amino Acid Requirements in Healthy Adult Human Subjects J. Nutr., January 1, 2006; 136(1): 256S - 263S. [Abstract] [Full Text] [PDF]

				S. Moehn, R. F. P. Bertolo, P. B. Pencharz, and R. O. Ball Development of the Indicator Amino Acid Oxidation Technique to Determine the Availability of Amino Acids from Dietary Protein in Pigs J. Nutr., December 1, 2005; 135(12): 2866 - 2870. [Abstract] [Full Text] [PDF]

				R. F. Bertolo, S. Moehn, P. B. Pencharz, and R. O. Ball Estimate of the variability of the lysine requirement of growing pigs using the indicator amino acid oxidation technique J Anim Sci, November 1, 2005; 83(11): 2535 - 2542. [Abstract] [Full Text] [PDF]

				C. C. Metges, K. J. Petzke, G. Backes, A. Elsner, P. Junghans, M. Derno, G. Nurnberg, and U. Hennig Response to lysine in a wheat gluten diet in adult minipigs after short-and long-term dietary adaptation as assessed with an indicator amino acid oxidation and balance technique J Anim Sci, April 1, 2005; 83(4): 833 - 841. [Abstract] [Full Text] [PDF]