QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jia, Q. Articles by Pestka, J. J. Search for Related Content PubMed PubMed Citation Articles by Jia, Q. Articles by Pestka, J. J.

---

Nutritional Immunology

Docosahexaenoic Acid Attenuates Mycotoxin-Induced Immunoglobulin A Nephropathy, Interleukin-6 Transcription, and Mitogen-Activated Protein Kinase Phosphorylation in Mice¹

Qunshan Jia^*,, Hui-Ren Zhou^*,, Maurice Bennink^* and James J. Pestka^*,**,2

^* Department of Food Science and Human Nutrition, Center for Integrative Toxicology, and ^** Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48823

²To whom correspondence should be addressed. E-mail: pestka{at}pilot.msu.edu.

The purpose of this investigation was to evaluate the dose-dependent effects of docosahexaenoic acid (DHA) on deoxynivalenol (DON)-induced IgA nephropathy in mice and their relation to proinflammatory gene expression and mitogen-activated protein kinase (MAPK) activation. Consumption of a modified AIN-93G diet containing 1, 5, and 30 g/kg DHA resulted in dose-dependent increases of DHA in liver phospholipids with concomitant decreases in arachidonic acid compared with control diets. DHA dose dependently inhibited increases in serum IgA and IgA immune complexes (IC) as well as IgA deposition in the kidney in DON-fed mice; the 30 g/kg DHA diet had the earliest detectable effects and maximal efficacy. Both splenic interleukin-6 (IL-6) mRNA and heterogeneous nuclear RNA (hnRNA), an indicator of IL-6 transcription, were significantly reduced in DON-fed mice that consumed 5 and 30 g/kg DHA; a similar reduction was observed for cyclooxygenase (COX-2) mRNA. In a subsequent study, acute DON exposure (25 mg/kg body weight) induced splenic IL-6 mRNA and hnRNA as well as COX-2 mRNA in mice fed the control diet, whereas induction of both RNA species was significantly inhibited in mice fed 30 g/kg DHA. These latter inhibitory effects corresponded to a reduction in DON-induced phosphorylation of p38, extracellular-signal related kinase 1/2, and c-Jun N-terminal kinase 1/2 MAPKs in the spleen. Taken together, the results indicate that DHA dose-dependently inhibited DON-induced IgA dysregulation and nephropathy, and that impairment of MAPK activation and expression of COX-2 and IL-6 are potential critical upstream mechanisms.

KEY WORDS: • IgA • interleukin 6 • deoxynivalenol • n-3 fatty acid • mitogen-activated protein kinase

This article has been cited by other articles:

				P. M. Ferraro, G. F. Ferraccioli, G. Gambaro, P. Fulignati, and S. Costanzi Combined treatment with renin-angiotensin system blockers and polyunsaturated fatty acids in proteinuric IgA nephropathy: a randomized controlled trial Nephrol. Dial. Transplant., January 1, 2009; 24(1): 156 - 160. [Abstract] [Full Text] [PDF]

				P. Balakumar, Vishal Arvind Chakkarwar, V. Kumar, A. Jain, J. Reddy, and M. Singh Experimental models for nephropathy Journal of Renin-Angiotensin-Aldosterone System, December 1, 2008; 9(4): 189 - 195. [Abstract] [PDF]

				E. Beli, M. Li, C. Cuff, and J. J. Pestka Docosahexaenoic Acid-Enriched Fish Oil Consumption Modulates Immunoglobulin Responses to and Clearance of Enteric Reovirus Infection in Mice J. Nutr., April 1, 2008; 138(4): 813 - 819. [Abstract] [Full Text] [PDF]

				R. J Deckelbaum, T. S Worgall, and T. Seo n-3 Fatty acids and gene expression Am. J. Clinical Nutrition, June 1, 2006; 83(6): S1520 - 1525S. [Abstract] [Full Text] [PDF]

				Q. Jia, H.-R. Zhou, Y. Shi, and J. J. Pestka Docosahexaenoic Acid Consumption Inhibits Deoxynivalenol-Induced CREB/ATF1 Activation and IL-6 Gene Transcription in Mouse Macrophages J. Nutr., February 1, 2006; 136(2): 366 - 372. [Abstract] [Full Text] [PDF]