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Nutrient Requirements

Leucine Supplementation Has an Anabolic Effect on Proteins in Rabbit Skin Wound and Muscle^1,2

Xiao-jun Zhang^*,, David L. Chinkes^*, and Robert R. Wolfe^*,,**,3

^* Metabolism Unit, Shriners Hospital for Children, Galveston, TX 77550 and Department of Surgery and ^** Department of Anesthesiology, University of Texas Medical Branch, Galveston, TX 77550

³To whom correspondence should be addressed. E-mail: rwolfe{at}utmb.edu.

We investigated the effect of leucine supplementation on protein metabolism in skin wounds and muscle in anesthetized rabbits. L-[ring-¹³C₆]phenylalanine was infused on d 7 after the ear was scalded, and the scalded ear and uninjured hindlimb were used as arteriovenous units to reflect protein kinetics in skin wounds and muscle. In comparison with a commercially available amino acid solution (10% Travasol), isonitrogenous [1638 µmol/(kg · h)] infusion of the amino acid solution with supplemental leucine to account for 35% of total nitrogen increased the net phenylalanine balance (P < 0.05) in the skin wound and muscle from –6.7 ± 6.1 to 0.9 ± 1.4 and from –4.4 ± 2.4 to –1.0 ± 0.4 µmol/(100 g · h), respectively. Infusion of leucine alone did not significantly improve the net phenylalanine balance in either skin wounds [–4.0 ± 4.6 µmol/(100 g · h)] or muscle [–2.7 ± 0.7 µmol/(100 g · h)]. We conclude that leucine supplementation had an anabolic effect on proteins in skin wounds and muscle, provided that adequate additional amino acids were also available.

KEY WORDS: • stable isotopes • gas chromatograph-mass spectrometer • fractional synthesis rate • arteriovenous balance

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