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Biochemical and Molecular Actions of Nutrients

Synthesis and Secretion of ApoC-I and ApoE during Maturation of Human SW872 Liposarcoma Cells¹

Hyperlipidemia and Atherosclerosis Research Group, Clinical Research Institute of Montréal, Montréal, QC, Canada

²To whom correspondence should be addressed. E-mail: cohnj{at}ircm.qc.ca.

Little is known about the regulation of apolipoprotein (apo) C-I production by human adipocytes. The aim of the present study, therefore, was to investigate the effect of different tissue culture conditions on the synthesis and secretion of apoC-I and apoE in human SW872 liposarcoma cells. After 3–4 d in culture (0.5 x 10⁶ cells/well, DMEM/F-12 medium with 10% fetal calf serum), cells reached confluence and became growth arrested. The molar ratio of apoE:apoC-I in the cell was 8.9 ± 0.6 and in the medium was 6.6 ± 0.5. After 17 d in culture, SW872 cells contained significantly more cholesterol (100%) and triglyceride (3-fold) and secreted more apoC-I [4 vs. 17 d: 0.11 ± 0.01 vs. 0.23 ± 0.01 pmol/(10⁶ cells · 24 h), P < 0.001] and apoE [0.7 ± 0.1 vs. 3.1 ± 0.3 pmol/(10⁶ cells · 24 h), P < 0.001]. Cellular apoC-I increased 7-fold and apoE increased 16-fold. Cell maturation was associated with significantly higher levels of apoE mRNA but not apoC-I mRNA. Increases in cell lipids, apoC-I, and apoE were not dependent on the presence of extracellular lipids because similar changes occurred in cells incubated with lipoprotein-deficient serum or in cells incubated without serum. Treatment (7 d) of cells during maturation with insulin (10 or 1000 nmol/L) significantly reduced the secretion of apoC-I and apoE. These results demonstrate that in maturing SW872 cells, cholesterol and triglyceride accumulation in the presence or absence of extracellular lipids, is associated with increased apoC-I and apoE production. Furthermore, apoC-I and apoE production are differentially regulated at the transcriptional level, and long-term treatment with insulin has an inhibitory rather than stimulatory effect on apoC-I and apoE production.

KEY WORDS: • adipose tissue • atherosclerosis • cholesterol • insulin • obesity

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