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© 2003 The American Society for Nutritional Sciences J. Nutr. 133:2084S-2089S, June 2003

Supplement: 2nd Amino Acid Workshop

Plasma Protein Synthesis Measurements Using a Proteomics Strategy1

H. M. H. van Eijk and N. E. P. Deutz2

Department of Surgery, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, 6200 MD Maastricht, The Netherlands

2 To whom correspondence should be addressed. E-mail: nep.deutz{at}ah.unimaas.nl.

The analysis of the synthesis of proteins has been the subject of many studies in animals and humans. Plasma proteins can be used as an easy accessible source of specific proteins. In this paper, an innovative method to study the synthetic rate of plasma proteins is described. This methodology, based on the proteomics approach, enables the direct observation of the effects of posttranslational modifications of protein synthesis and/or degradation. The methodology is based on 1D or 2D electrophoresis and subsequent electrospray ionization liquid chromatography mass spectrometry (ESI-LC-MS). Protein synthesis is measured in isotopically labeled peptides of the identified proteins. This innovative method can be used to assess amino acid adequacy and safety by studying protein synthesis and posttranslational modification of plasma proteins in more detail.

KEY WORDS: • plasma proteins • proteomics • mass spectrometry • stable isotopes




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