QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ferrer, C. Articles by Moretó, M. Search for Related Content PubMed PubMed Citation Articles by Ferrer, C. Articles by Moretó, M.

Dietary Lipids Modify Brush Border Membrane Composition and Nutrient Transport in Chicken Small Intestine

Carmen Ferrer, Elvira Pedragosa, Mònica Torras-Llort^*, Xavier Parcerisa, Magda Rafecas, Ruth Ferrer^*, Concepció Amat^* and Miquel Moretó^*,5

Centre Especial de Recerca en Nutrició i Ciència dels Aliments, ^* Departament de Fisiologia-Divisió IV and Departament de Nutrició i Bromatologia-CeRTA, Universitat de Barcelona, Barcelona, Spain

⁵To whom correspondence should be addressed. E-mail: mmoreto{at}farmacia.far.ub.es.

The influence of dietary fatty acids (FA) on intestinal brush border FA composition and nutrient transport functions was studied in broiler chickens. Ross chicks (2 wk old) were fed for 14 d a standard diet (CTL) or diets enriched with saturated fatty acids (SFA; 60 g/kg lard, LAR diet), (n-3) PUFA (60 g/kg linseed oil, LSO diet) and (n-6) PUFA (60 g/kg sunflower oil, SFO diet). The SFA of the brush border membrane were within 40–44% of total FA in spite of wide variability in dietary SFA concentration (13–32%); membrane (n-6) and (n-3) PUFA strongly reflected their dietary intake and thus the (n-6)/(n-3) ratio. However, the membrane polyunsaturated/saturated ratio (P/S) was close to unity, whereas in the diets, it was between 0.9 and 5. The transport kinetic constants (V_max, K_m, K_d) of D-glucose (substrate of the sodium glucose cotransporter 1), L-lysine (through systems b^0,+ and y⁺_m) and L-methionine (through systems B and L) were studied in jejunal brush border membrane vesicles. The changes in dietary FA intake did not affect the K_m of the substrates for their transporters. Both LAR and SFO diets reduced the D-glucose V_max, which was compensated for by an increase in the K_d. The LAR diet reduced lysine transport across y⁺_m, whereas the LSO diet increased the V_max for both lysine and methionine.

KEY WORDS: • jejunum • glucose • lysine • methionine • fatty acids • chickens

This article has been cited by other articles:

				S. Roig-Perez, F. Guardiola, M. Moreto, and R. Ferrer Lipid peroxidation induced by DHA enrichment modifies paracellular permeability in Caco-2 cells: protective role of taurine J. Lipid Res., August 1, 2004; 45(8): 1418 - 1428. [Abstract] [Full Text] [PDF]