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Supplement: Biomarkers of Nutritional Exposure and Nutritional Status

Urine Nitrogen as a Biomarker for the Validation of Dietary Protein Intake¹

Medical Research Council, Cambridge, U.K.

² To whom correspondence should be addressed. E-mail: sab{at}mrc-dunn.cam.ac.uk.

When validated for completeness, 24-h urine nitrogen obtained from repeated 24-h urine collections has provided useful insights into the validity of dietary assessments, underreporting behaviors and the structure of measurement errors that are associated with different methods. This is particularly so when nitrogen is combined with another marker in 24-h urine samples, potassium. Although the collection of 24-h urine is a tedious procedure, the method is readily accessible and comparatively inexpensive. Other markers of dietary intake and intermediate risk markers may also be measured in the 24-h urine that is obtained.

KEY WORDS: • urine nitrogen • dietary assessments • biomarkers • protein • epidemiology

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