![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Food Research Institute, Department of Food Microbiology and Toxicology, University of Wisconsin-Madison, Madison, WI 53706
4To whom correspondence should be addressed. E-mail: mwpariza{at}facstaff.wisc.edu.
Feeding diets supplemented with t10c12 conjugated linoleic acid (CLA) to growing mice reduces body fat mass. The effects are evident after 1 wk and maximal by 3 wk and are accompanied by reductions in fat cell size. This may complicate direct comparisons with adipocytes from control mice. Accordingly, we investigated the early biochemical events that occur within adipocytes during the first week of CLA feeding, before changes in the size of adipocytes have occurred. Female ICR mice were fed a control diet or a diet supplemented with 0.5 g/100 g of CLA for 4 d, at which time there were no differences in body weight, fat mass or adipocyte size (except that CLA-fed mice had fewer adipocytes >90 µm in diameter). Parametrial adipose tissue from the CLA-fed mice had significantly reduced heparin-releasable lipoprotein lipase (LPL) and intracellular LPL activities and significantly reduced glucose incorporation into CO2, fatty acid and glycerol. There were no differences between adipose tissues from CLA-fed or control mice in the ratios of 16:0 to 16:1 and 18:0 to 18:1 fatty acids or in norepinephrine-stimulated lipolysis. Serum insulin levels in food-deprived mice, measured at 4 d and 7 wk, did not differ between groups nor did the concentration of free fatty acids in serum of food-deprived or fed mice measured at the same time points. In mice, CLA-induced inhibition of heparin-releasable LPL and glucose metabolism may be the most important early steps leading to subsequent body fat reduction. In addition, CLA does not appear to enhance lipolysis in mouse adipose tissue in vivo.
KEY WORDS: • conjugated linoleic acid • lipoprotein lipase • lipolysis • glucose metabolism
This article has been cited by other articles:
|
|
 |
|
  L.-F. Liu, A. Purushotham, A. A. Wendel, and M. A. Belury Combined effects of rosiglitazone and conjugated linoleic acid on adiposity, insulin sensitivity, and hepatic steatosis in high-fat-fed mice Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1671 - G1682. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C. LaRosa, J. Miner, Y. Xia, Y. Zhou, S. Kachman, and M. E. Fromm Trans-10, cis-12 conjugated linoleic acid causes inflammation and delipidation of white adipose tissue in mice: a microarray and histological analysis Physiol Genomics, November 21, 2006; 27(3): 282 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. House, J. P. Cassady, E. J. Eisen, T. E. Eling, J. B. Collins, S. F. Grissom, and J. Odle Functional genomic characterization of delipidation elicited by trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) in a polygenic obese line of mice Physiol Genomics, May 11, 2005; 21(3): 351 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. Mirand, M.-A. Arnal-Bagnard, L. Mosoni, Y. Faulconnier, J.-M. Chardigny, and Y. Chilliard Cis-9, Trans-11 and Trans-10, Cis-12 Conjugated Linoleic Acid Isomers Do Not Modify Body Composition in Adult Sedentary or Exercised Rats J. Nutr., September 1, 2004; 134(9): 2263 - 2269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W Pariza Perspective on the safety and effectiveness of conjugated linoleic acid Am. J. Clinical Nutrition, June 1, 2004; 79(6): 1132S - 1136S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Cianflone, R. Zakarian, C. Couillard, B. Delplanque, J.-P. Despres, and A. Sniderman Fasting acylation-stimulating protein is predictive of postprandial triglyceride clearance J. Lipid Res., January 1, 2004; 45(1): 124 - 131. [Abstract] [Full Text] [PDF]
|
|
