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Biochemical and Molecular Actions of Nutrients

Fish Oil Affects Pancreatic Fat Storage, Pyruvate Dehydrogenase Complex Activity and Insulin Secretion in Rats Fed a Sucrose-Rich Diet^1,2

Department of Biochemistry, School of Biochemistry, University of Litoral, Santa Fe, Argentina and ^* Endocrinology Research Center, Hospital Ricardo Gutierrez, Buenos Aires, Argentina

³To whom correspondence should be addressed. E-mail: ylombard{at}fbcb.unl.edu.ar.

Rats fed a sucrose-rich diet (SRD) develop hypertriglyceridemia and a marked decline in ß cell function. The purpose of this study was to determine whether changes in triglyceride concentration and/or altered pyruvate dehydrogenase complex (PDHc) activity contribute to the ß cell dysfunction, and to analyze the effect of dietary fish oil on the altered patterns of insulin secretion and peripheral insulin resistance. Rats were fed an SRD for 210 d. One-half of the rats continued consuming the SRD until d 270. The other half received an SRD in which fish oil (FO) was partially substituted for corn oil until d 270. A group of rats was fed a control diet (CD) throughout the experiment. The islets of rats fed the SRD had a greater triglyceride concentration and lower PDHc activity than those fed the CD. Insulin secretion patterns under the stimulus of glucose, palmitate or L-arginine were impaired in SRD-fed compared with CD-fed rats. This was accompanied by peripheral insulin resistance, mild hyperglycemia, a sharp increase of plasma triglyceride and free fatty acid levels and greater epididymal and retroperitoneal fat weights. FO normalized and/or improved these variables. Our results indicate that the increased fat storage and decreased PDHc activity in the ß cells play a key role in the abnormal insulin secretion of rats chronically fed an SRD. This is consistent with the reversion of these alterations by dietary FO.

KEY WORDS: • dyslipemia • ß cell function • fish oil • sucrose-rich diet

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