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Biochemical and Molecular Actions of Nutrients

Conjugated Linoleic Acids Lower the Release of Eicosanoids and Nitric Oxide from Human Aortic Endothelial Cells¹

Institut für Ernährungswissenschaften, Martin-Luther-Universität Halle-Wittenberg, D-06108 Halle/Saale, Germany

²To whom correspondence should be addressed. E-mail: eder{at}landw.uni-halle.de.

The objective of this study was to determine the effects of cis-9, trans-11 and trans-10, cis-12 CLA on the release of vasoactive eicosanoids and nitric oxide (NO) in human aortic endothelial cells. Experiments were conducted in which cells were incubated with these fatty acids, and the concentrations of various eicosanoids [6-keto prostaglandin (PG) F₁ as a stable product of PGI₂, thromboxane (TX) B₂ as a stable product of TXA₂, and PGE₂] and NO in the medium were determined. Cells treated with 50 µmol/L of either cis-9, trans-11 or trans-10, cis-12 CLA released less of all of the eicosanoids and NO than control cells treated with medium alone (P < 0.05). The ratio between the amounts of 6-keto-PGF₁ and that of TXB₂ released did not differ between control cells and cells treated with either CLA isomer. Moreover, cells treated with 50 µmol/L of cis-9, trans-11 or trans-10, cis-12 CLA had a lower amount of arachidonic acid in their phosphatidylethanolamine fraction and a lower mRNA concentration and activity of secretory phospholipase A₂ than control cells (P < 0.05). These data suggest that eicosanoid formation was impaired by a reduced availability of arachidonic acid for the cyclooxygenase pathway. In conclusion, this study shows that cis-9, trans 11-CLA and trans-10, cis-12 CLA influence the release of various eicosanoids and NO from human aortic endothelial cells. The effects observed in this study might be important because eicosanoids and NO released from endothelial cells are involved in the regulation of vessel tone and platelet aggregation. The results of the present study suggest that both CLA isomers had unfavorable effects on endothelial function.

KEY WORDS: • conjugated linoleic acids • endothelial cell • eicosanoids • nitric oxide

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