![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Nutrition Research Division, Food Directorate, Health Products and Food Branch, Health Canada, Ottawa, Ontario, Canada
2To whom correspondence should be addressed. E-mail: mary_l'abbe{at}hc-sc.gc.ca
The most commonly used indices for determining copper deficiency in humans are reduced serum/plasma copper concentration and decreased activity of ceruloplasmin and Cu/Zn superoxide dismutase (SOD1). However, these indicators are influenced by many factors unrelated to copper status and lack the sensitivity required to detect marginal deficiency, limiting their usefulness in many situations. In vivo, the insertion of copper into SOD1 is dependent on the copper chaperone for SOD1 (CCS). In this study, we explored the possibility that the expression level of CCS may reflect copper status and thus serve as a useful marker of copper nutriture. Weanling male Wistar rats were fed either a normal (5.3 mg Cu/kg diet), moderately deficient (0.84 mg Cu/kg diet) or deficient (0.34 mg Cu/kg diet) copper diet for 6 wk. Rats fed moderate and deficient diets showed differences (P < 0.05) in several hematological measurements, indicating varying degrees of copper deficiency in these groups. Copper-deficient rats had reduced (P < 0.05) liver and erythrocyte SOD1 activity and body weight. Western blot analysis revealed a dose-dependent increase (P < 0.05) in CCS expression in liver and erythrocytes of copper-deficient rats. We report CCS protein level as a novel marker for assessing copper status.
KEY WORDS: • copper status • copper chaperone • Cu/Zn superoxide dismutase • expression level • rats
This article has been cited by other articles:
|
|
 |
|
  G. M. Hill and J. E. Link Transporters in the absorption and utilization of zinc and copper J Anim Sci, April 1, 2009; 87(14_suppl): E85 - E89. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Olivares, M. A Mendez, P. A Astudillo, and F. Pizarro Present situation of biomarkers for copper status Am. J. Clinical Nutrition, September 1, 2008; 88(3): 859S - 862S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Turski and D. J. Thiele Drosophila Ctr1A Functions as a Copper Transporter Essential for Development J. Biol. Chem., August 17, 2007; 282(33): 24017 - 24026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, Q. Liu, Q. Chen, N.-Q. Liu, F.-L. Li, Z.-B. Lu, C. Qin, H. Zhu, Y.-Y. Huang, W. He, et al. Nicotine attenuates {beta}-amyloid-induced neurotoxicity by regulating metal homeostasis FASEB J, June 1, 2006; 20(8): 1212 - 1214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Caruano-Yzermans, T. B. Bartnikas, and J. D. Gitlin Mechanisms of the Copper-dependent Turnover of the Copper Chaperone for Superoxide Dismutase J. Biol. Chem., May 12, 2006; 281(19): 13581 - 13587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Chen, G. Huang, T. Su, H. Gao, Z. K. Attieh, A. T. McKie, G. J. Anderson, and C. D. Vulpe Decreased Hephaestin Activity in the Intestine of Copper-Deficient Mice Causes Systemic Iron Deficiency J. Nutr., May 1, 2006; 136(5): 1236 - 1241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. West and J. R. Prohaska Cu,Zn-Superoxide Dismutase Is Lower and Copper Chaperone CCS Is Higher in Erythrocytes of Copper-Deficient Rats and Mice Experimental Biology and Medicine, September 1, 2004; 229(8): 756 - 764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Prohaska and A. A. Gybina Intracellular Copper Transport in Mammals J. Nutr., May 1, 2004; 134(5): 1003 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chung, J. R. Prohaska, and M. Wessling-Resnick Ferroportin-1 Is Not Upregulated in Copper-Deficient Mice J. Nutr., March 1, 2004; 134(3): 517 - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Gybina and J. R. Prohaska Increased Rat Brain Cytochrome C Correlates with Degree of Perinatal Copper Deficiency Rather than Apoptosis J. Nutr., November 1, 2003; 133(11): 3361 - 3368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bertinato and M. R. L'Abbe Copper Modulates the Degradation of Copper Chaperone for Cu,Zn Superoxide Dismutase by the 26 S Proteosome J. Biol. Chem., September 12, 2003; 278(37): 35071 - 35078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Prohaska, J. Geissler, B. Brokate, and M. Broderius Copper, Zinc-Superoxide Dismutase Protein but not mRNA Is Lower in Copper-Deficient Mice and Mice Lacking the Copper Chaperone for Superoxide Dismutase Experimental Biology and Medicine, September 1, 2003; 228(8): 959 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. B. Bartnikas and J. D. Gitlin Mechanisms of Biosynthesis of Mammalian Copper/Zinc Superoxide Dismutase J. Biol. Chem., August 29, 2003; 278(35): 33602 - 33608. [Abstract] [Full Text] [PDF]
|
|
