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Nutrition and Cancer

Dietary Inulin Suppresses Azoxymethane-Induced Aberrant Crypt Foci and Colon Tumors at the Promotion Stage in Young Fisher 344 Rats¹

Nutrition and Carcinogenesis Laboratory, Department Food and Animal Sciences, Alabama A&M University, Normal, AL 35762

²To whom correspondence should be addressed. E-mail: mverghese{at}aamu.edu

This study was designed to determine the effect of 10% dietary long-chain inulin on the azoxymethane (AOM)-induced colonic preneoplastic aberrant crypt foci (ACF) and small intestinal and colon tumors at the initiation (I), promotion (P) and I + P stages (20 rats per treatment) in Fisher 344 male weanling rats. After an acclimatization period of 1 wk, groups of Fisher 344 male weanling rats were assigned to consume AIN 93G diet (control) or AIN 93G diet containing 10% inulin. All the rats received 16 mg/kg body AOM dissolved in saline subcutaneously at 7 wk of age followed by a second injection at 8 wk of age. An additional group of five rats received only saline and consumed the control diet. The rats received the assigned diets until asphyxiation by CO₂ at 16 wk of age for the ACF experiment and 45 wk for the end-point tumor experiment. Feed intake, weight gain, diarrheal index, cecal weight, cecal pH, ACF and tumors in the colon were determined. Rats fed inulin had diarrhea after 2 wk of feeding and recovered by 4 wk. Cecal weight was greater in rats fed inulin and cecal pH was lower. The inulin group had more than 66% fewer aberrant crypts and 60% fewer ACF compared with the control group. Tumor incidences in the small intestine and colon of rats in the control, I, P and I + P groups were: 78, 31, 0 and 11% and 90, 73, 69 and 50%, respectively. The corresponding values for the distal portion of the colon were 87, 63, 45 and 33%, respectively. Colon tumors per tumor-bearing rat were 4.2, 3.09, 1.36 and 1.2 for the control, I, P and I + P groups, respectively. All groups differed, P < 0.05. The results of this study indicate that dietary long-chain inulin suppresses AOM-induced ACF formation, an early preneoplastic marker of colon tumorigenesis in rats, and colon tumors, particularly at the promotion stage.

KEY WORDS: • azoxymethane • aberrant crypt foci • inulin • colon tumors • Fisher 344 rats

This article has been cited by other articles:

				M. Verghese, D. R. Rao, C. B. Chawan, and L. Shackelford Dietary Inulin Suppresses Azoxymethane-Induced Preneoplastic Aberrant Crypt Foci in Mature Fisher 344 Rats J. Nutr., September 1, 2002; 132(9): 2804 - 2808. [Abstract] [Full Text] [PDF]