![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Departments of Nutrition Sciences, Medicine and Pediatrics, Divisions of Biochemistry and Gastroenterology, University of Alabama at Birmingham, Birmingham, AL 35233
3To whom correspondence should be addressed. E-mail: ndashti{at}uab.edu.
The objective of this study was to assess the relative long-term effects of linoleic (cis, cis 18:2), linolelaidic (trans, trans 18:2), and palmitic (16:0) acids on hepatic lipoprotein production in HepG2 cells. All fatty acids increased the mass of triglycerides (TG) in the medium and the incorporation of [3H]-glycerol into secreted TG; the increase was more pronounced with linoleic acid than with linolelaidic and palmitic acids. The net accumulation in the medium of apolipoprotein (apo) A-I was not affected by the fatty acids tested and moderate changes in that of apoB resulted in apoB/apoA-I mass ratios of 1.05, 1.27 and 0.86 with linoleic, linolelaidic and palmitic acids, respectively. The incorporation of [14C]-acetate into cellular plus secreted total sterols was 9.1%, 33.6% and 17.4% of total [14C]-labeled lipids with linoleic, linolelaidic and palmitic acids, respectively. Relative to linoleic acid, palmitic acid, and to a greater extent (P < 0.05) linolelaidic acid, increased the secretion and cellular accumulation of [14C]-labeled free cholesterol (FC) and cholesteryl esters and decreased those of TG and phospholipids (PL). Compared with linoleic acid, linolelaidic acid increased LDL-cholesterol (C) and HDL-C by 154% (P < 0.001) and 50% (P = 0.016), respectively, whereas palmitic acid increased LDL-C by 17% (P > 0.1) and did not affect HDL-C. The LDL-C to HDL-C ratios were 0.70, 1.18 and 0.96 with linoleic, linolelaidic and palmitic acids, respectively. These differences were not due to altered LDL receptor activity. The PL to C ratios of HDL particles were 1.61, 0.40 and 0.77 with linoleic acid, linolelaidic acid and palmitic acid, respectively. These results suggest that relative to cis polyunsaturated and saturated fatty acids, trans PUFA more adversely affect the concentration and composition of apoA-I- and apoB-containing lipoproteins secreted by HepG2 cells.
KEY WORDS: • cholesterol metabolism • triglyceride secretion • liver • LDL • HDL
This article has been cited by other articles:
|
|
 |
|
  M. Manchekar, P. E. Richardson, Z. Sun, Y. Liu, J. P. Segrest, and N. Dashti Charged Amino Acid Residues 997-1000 of Human Apolipoprotein B100 Are Critical for the Initiation of Lipoprotein Assembly and the Formation of a Stable Lipidated Primordial Particle in McA-RH7777 Cells J. Biol. Chem., October 24, 2008; 283(43): 29251 - 29265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Dashti, M. Manchekar, Y. Liu, Z. Sun, and J. P. Segrest Microsomal Triglyceride Transfer Protein Activity Is Not Required for the Initiation of Apolipoprotein B-containing Lipoprotein Assembly in McA-RH7777 Cells J. Biol. Chem., September 28, 2007; 282(39): 28597 - 28608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N Dashti, G McGwin, C Owsley, and C A Curcio Plasma apolipoproteins and risk for age related maculopathy Br J Ophthalmol, August 1, 2006; 90(8): 1028 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mozaffarian, M. B. Katan, A. Ascherio, M. J. Stampfer, and W. C. Willett Trans Fatty Acids and Cardiovascular Disease N. Engl. J. Med., April 13, 2006; 354(15): 1601 - 1613. [Full Text] [PDF]
|
|
|
|
 |
|
 E. E Mosley, A. L Wright, M. K McGuire, and M. A McGuire trans Fatty acids in milk produced by women in the United States Am. J. Clinical Nutrition, December 1, 2005; 82(6): 1292 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Dashti, G. Datta, M. Manchekar, M. Chaddha, and G. M. Anantharamaiah Model class A and class L peptides increase the production of apoA-I-containing lipoproteins in HepG2 cells J. Lipid Res., October 1, 2004; 45(10): 1919 - 1928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Manchekar, P. E. Richardson, T. M. Forte, G. Datta, J. P. Segrest, and N. Dashti Apolipoprotein B-containing Lipoprotein Particle Assembly: LIPID CAPACITY OF THE NASCENT LIPOPROTEIN PARTICLE J. Biol. Chem., September 17, 2004; 279(38): 39757 - 39766. [Abstract] [Full Text] [PDF]
|
|
