QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Coëffier, M. Articles by Déchelotte, P. Search for Related Content PubMed PubMed Citation Articles by Coëffier, M. Articles by Déchelotte, P.

---

Nutrient-Gene Interactions
Research Communication

Acute Enteral Glutamine Infusion Enhances Heme Oxygenase-1 Expression in Human Duodenal Mucosa¹

Appareil Digestif, Environnement et Nutrition (ADEN, EA 3234) and Institut Fédératif de Recherches Multidisciplinaires sur les peptides (IFR n° 23), Faculté de Médecine-Pharmacie, 76183 Rouen Cedex and ^* Service d’Anatomie Pathologique, Hôpital Charles Nicolle, Rouen, France

²To whom correspondence should be addressed. E-mail: Pierre.Dechelotte{at}chu-rouen.fr.

The heat shock protein, heme oxygenase-1 (HO-1), contributes to the protection of the intestine. Some experimental models suggest that induction of HO-1 by glutamine may contribute to the preservation of intestinal mucosa. The effect of an enteral infusion of glutamine for 6 h on HO-1 expression in duodenal mucosa was studied in healthy men and women and compared with an isonitrogenous mixture of amino acids. After enteral infusion, endoscopic duodenal biopsies were performed and either fixed in formalin for immunohistochemistry or frozen for HO-1 mRNA analysis by reverse transcriptase-polymerase chain reaction. Histologic examination revealed that HO-1 was constitutively expressed in intestinal epithelial cells (IEC), and that glutamine increased the grade of HO-1 immunostaining (P 0.01). Glutamine also increased the percentage of HO-1 immunoreactive lamina propria cells (LPC, 10.5 vs. 7.5%, P 0.05). Glutamine significantly increased HO-1 mRNA expression compared with control amino acids: median (range) 156 (102–182) vs. 100 (68–179)%, P 0.05. The mRNA level for HO-1 was correlated with the percentage of immunoreactive LPC (r = 0.55, P = 0.017) and the grade of immunostaining in IEC (r = 0.51, P = 0.030). In conclusion, glutamine enhanced HO-1 mRNA and protein expression in human duodenal mucosa. These data support further evaluation of the effects of glutamine on intestinal HO-1 during states of intestinal inflammation.

KEY WORDS: • glutamine • heme oxygenase-1 • heat shock protein • intestine • humans

This article has been cited by other articles:

				M. McQuiggan, R. Kozar, R. M. Sailors, C. Ahn, B. McKinley, and F. Moore Enteral Glutamine During Active Shock Resuscitation Is Safe and Enhances Tolerance of Enteral Feeding JPEN J Parenter Enteral Nutr, January 1, 2008; 32(1): 28 - 35. [Abstract] [Full Text] [PDF]

				N Gassler, D Newrzella, C Bohm, S Lyer, L Li, O Sorgenfrei, L van Laer, B Sido, J Mollenhauer, A Poustka, et al. Molecular characterisation of non-absorptive and absorptive enterocytes in human small intestine Gut, August 1, 2006; 55(8): 1084 - 1089. [Abstract] [Full Text] [PDF]

				A. Hubert-Buron, J. Leblond, A. Jacquot, P. Ducrotte, P. Dechelotte, and M. Coeffier Glutamine Pretreatment Reduces IL-8 Production in Human Intestinal Epithelial Cells by Limiting I{kappa}B{alpha} Ubiquitination J. Nutr., June 1, 2006; 136(6): 1461 - 1465. [Abstract] [Full Text] [PDF]