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Nutrient-Gene Interactions

Weaning Affects the Expression of Heat Shock Proteins in Different Regions of the Gastrointestinal Tract of Piglets

Jean Claude David^*1, J. F. Grongnet^* and J. P. Lalles^*,

^* Ecole Nationale Supérieure Agronomique de Rennes, Rennes Cedex, France and Institut National de la Recherche Agronomique, Unité Mixte de Recherches sur le Veau et le Porc, Saint Gilles, France

¹To whom correspondence should be addressed. E-mail: david{at}roazhon.inra.fr.

Heat shock proteins (HSP) play a central role in the protection of cells, tissues or organs subjected to various types of stressors. Different nutrients have been recently shown to exert their protection through the induction of HSP. Because these nutrients alleviate alterations of the intestine after weaning in pigs, this study was designed to obtain basic information on the expression of HSP 27, heat shock cognate 70 (HSC 70), HSP 70 and HSP 90 along the gastrointestinal tract (GIT) of young pigs and to study the effect of weaning on this expression. Pigs were weaned at 28 or 21 d and slaughtered at various times postweaning. All HSP were expressed in the GIT segments studied before and after weaning. However, the expression of HSP 27 and HSP 70 was transiently increased in the stomach and duodenum between 6 and 12 h postweaning and between 24 and 48 h in the mid-jejunum, ileum and colon. Their expressions were transiently decreased in the ileum. Expression of HSP 90 increased in the stomach and jejunum but decreased in the duodenum, ileum and colon. Similar results were obtained at both ages of weaning. We conclude that the HSP studied are present all along the gut of pigs and that their expression is modulated through weaning according to spatial-temporal patterns. The modulation by nutrients of HSP and their protective role on the GIT remain to be investigated in pigs.

KEY WORDS: • heat shock proteins • weaning • piglet