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Supplement: Trans-HHS Workshop: Diet, DNA Methylation Processes and Health

Applications of CpG Island Microarrays for High-Throughput Analysis of DNA Methylation^{1 ,2}

Department of Pathology and Anatomical Sciences, Ellis Fischel Cancer Center, University of Missouri, Columbia, MO 65203 and ^* Department of Zoology, Life Science College, National Chung Hsing University, Taichung 402, Taiwan, Republic of China

³To whom correspondence should be addressed. E-mail: huangh{at}health.missouri.edu.

Differential methylation hybridization (DMH) is a high-throughput microarray technique designed to identify changes in DNA methylation patterns commonly observed in cancer and other disease states. The DMH methodology comprises three fundamental components: the arraying of CpG island clones on glass slides, the preparation of the sample amplicons under investigation, and the hybridization of amplicon targets onto the CpG island microarray. Herein, we outline the DMH protocol and illustrate its utility and the validation approaches used in analyzing the hypermethylation profile of breast tumor and normal samples.

KEY WORDS: • DNA methylation • CpG islands • microarray

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