QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by O’Connor, T. P. Articles by Banz, W. J. Search for Related Content PubMed PubMed Citation Articles by O’Connor, T. P. Articles by Banz, W. J.

---

Nutritional Immunology

A High Isoflavone Soy Protein Diet and Intravenous Genistein Delay Rejection of Rat Cardiac Allografts¹

Timothy P. O’Connor², Daniel A. Liesen^*, Paul C. Mann, Lori Rolando and William J. Banz

Department of Surgery, Southern Illinois University School of Medicine, Springfield, IL 62702; ^* Department of Surgery, Medical College of Ohio, Toledo, OH 43614; and Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL 62901

²To whom correspondence should be addressed. E-mail: toconnor{at}siumed.edu.

Genistein, a soy isoflavone, has in vitro immunosuppressive properties. We investigated whether genistein or dietary soy protein containing isoflavones could influence the outcome of rat cardiac allografts. Lewis rats were fed a diet with protein from high isoflavone soy protein fraction (HIS), casein (CAS) or casein with isoflavones added (CI) starting 1 wk before heart transplants from Wistar Furth donors, and continuing throughout the study. HIS-fed rats had significantly prolonged time to rejection compared with CAS- and CI-fed recipients (10.8 ± 2.62 vs. 7.18 ± 0.75 and 7.22 ± 0.44 d, P < 0.001). Intravenous genistein [20mg/(kg · d) for 14 d] significantly prolonged heart survival compared with controls and dissolvent-treated recipients (23.2 ± 7.4 vs. 8.4 ± 1.3 and 11.4+/3.6 d, P < 0.0005), and had an additive effect when given to heart recipients also receiving low dose cyclosporine for 7 d (30.8 ± 2.3 vs. 23.4 ± 2.4 d, P < 0.005). Concanavalin A–stimulated lymphocytes, isolated from Lewis rats given intraperitoneal genistein for 7 d, had decreased production of interferon compared with controls or dimethyl sulfoxide–treated groups (22.6 ± 9.9 vs 149 ± 105 and 154 ± 103 µg/L, P < 0.05). In conclusion, a high isoflavone soy diet and intravenous genistein, but not isoflavone extract alone, delay rejection of rat cardiac allografts, with an additive effect in cyclosporine-treated rats. In addition, intraperitoneal genistein has immunosuppressive properties in vivo.

KEY WORDS: • soy • genistein • allograft • rejection • isoflavone • rats

This article has been cited by other articles:

				P. S. Cooke, V. Selvaraj, and S. Yellayi Genistein, Estrogen Receptors, and the Acquired Immune Response J. Nutr., March 1, 2006; 136(3): 704 - 708. [Abstract] [Full Text] [PDF]

				V. Selvaraj, D. Bunick, C. Finnigan-Bunick, R. W. Johnson, H. Wang, L. Liu, and P. S. Cooke Gene Expression Profiling of 17{beta}-Estradiol and Genistein Effects on Mouse Thymus Toxicol. Sci., September 1, 2005; 87(1): 97 - 112. [Abstract] [Full Text] [PDF]

				O. Mezei, W. J. Banz, R. W. Steger, M. R. Peluso, T. A. Winters, and N. Shay Soy Isoflavones Exert Antidiabetic and Hypolipidemic Effects through the PPAR Pathways in Obese Zucker Rats and Murine RAW 264.7 Cells J. Nutr., May 1, 2003; 133(5): 1238 - 1243. [Abstract] [Full Text] [PDF]