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Nutrient Metabolism

Legume Grains Enhance Ileal Losses of Specific Endogenous Serine-Protease Proteins in Weaned Pigs¹

Paulo Salgado^*, Lucile Montagne^*, João P. B. Freire, Ricardo B. Ferreira^,**, Artur Teixeira, Ofélia Bento, Manuel C. Abreu, René Toullec^* and Jean-Paul Lallès^*2

^* Unité Mixte de Recherche sur le Veau et le Porc, INRA-ENSA de Rennes, 35042 Rennes Cedex, France; Instituto Superior de Agronomia, 1349-017 Lisboa, Portugal; ^** Instituto de Tecnologia Química e Biológica, 2781-901 Oeiras, Portugal; and Departamento de Zootecnia, Universidade de Évora, 7002-554 Évora, Portugal

²To whom correspondence should be addressed. E-mail: lalles{at}roazhon.inra.fr.

Feeding legume grains to pigs usually increases losses of endogenous proteins at the terminal ileum. However, the identity of such proteins is largely unknown. This study was undertaken to determine the ileal flow and identity of soluble proteins present in large concentrations in ileal digesta of young pigs fed soybean meal (SBM), peas (P), faba beans (FB), or blue lupin (L) in expt. 1, and white (WPC) or black (BPC) chickpeas in expt. 2. Protein in the control diet (C) was provided by casein. Ileal digesta proteins were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis, Coomassie blue staining, densitometry and N-terminal amino acid sequencing. Three protein bands at molecular masses of 25, 27, and 30 kDa had a higher ileal flow (P < 0.05) in the pigs fed the legume-based diets compared to those fed the control diet in expt. 2. This was true for the 25- and 30-kDa proteins (P < 0.05) and the 27-kDa protein (P < 0.10) in pigs fed the legume-containing diets in expt. 1. These proteins shared N-terminal amino acid sequences with enzymes of the serine protease family including pig trypsin (25 kDa) and blood coagulation factor IX or chymotrypsin (27 and 30 kDa).

KEY WORDS: • piglets • endogenous proteins • serine-protease • proteins • densitometry • sequence analysis

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