QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Aschenbach, J. R. Articles by Gäbel, G. Search for Related Content PubMed PubMed Citation Articles by Aschenbach, J. R. Articles by Gäbel, G.

---

Nutrient Metabolism
Research Communication

Glucose Uptake via SGLT-1 Is Stimulated by ß₂-Adrenoceptors in the Ruminal Epithelium of Sheep^{1 ,2}

Department of Veterinary Physiology, Leipzig University, D-04103 Leipzig, Germany

³To whom correspondence should be addressed. E-mail: aschenb{at}rz.uni-leipzig.de.

Glucose absorption via the sodium glucose-linked transporter (SGLT)-1, decreases the glucose concentration in the ruminant forestomach and may ameliorate or prevent ruminal lactic acidosis. Because acidotic ruminants show increased sympathetic activity, the possibility of adrenergic modulation of SGLT-1 was investigated. Glucose uptake into ovine ruminal epithelia was measured in Ussing chambers after the addition of 200 µmol/L ¹⁴C-labeled glucose to the mucosal solution. Glucose uptake decreased (P < 0.05) by >50% in comparison with control after mucosal addition of the SGLT-1 inhibitor, phlorizin (100 µmol/L). Serosal preincubation with 100 µmol/L epinephrine increased (P < 0.05) the phlorizin-sensitive glucose uptake in the absence and presence of indomethacin (10 µmol/L). The effect of epinephrine was simulated by ß- (100 µmol/L isoproterenol) and ß₂-receptor agonists (10 µmol/L terbutaline), as well as by direct stimulation of adenylyl cyclase (10 µmol/L forskolin). The serosal addition of methoxamine, clonidine, dobutamine or BRL 37344 had no effect. Inhibition of protein kinase A with 2 µmol/L H 89 completely abolished the stimulation of glucose uptake by epinephrine. We conclude that ruminal SGLT-1 can be stimulated via ß₂-dependent generation of cyclic adenosine monophosphate.

KEY WORDS: • glucose absorption • cAMP • epinephrine • rumen • sheep

This article has been cited by other articles:

				G. B. Penner, J. R. Aschenbach, G. Gabel, R. Rackwitz, and M. Oba Epithelial Capacity for Apical Uptake of Short Chain Fatty Acids Is a Key Determinant for Intraruminal pH and the Susceptibility to Subacute Ruminal Acidosis in Sheep J. Nutr., September 1, 2009; 139(9): 1714 - 1720. [Abstract] [Full Text] [PDF]

				J. R. Aschenbach, S. Bilk, G. Tadesse, F. Stumpff, and G. Gabel Bicarbonate-dependent and bicarbonate-independent mechanisms contribute to nondiffusive uptake of acetate in the ruminal epithelium of sheep Am J Physiol Gastrointest Liver Physiol, May 1, 2009; 296(5): G1098 - G1107. [Abstract] [Full Text] [PDF]

				W. A. Awad, J. R. Aschenbach, F. M. C. S. Setyabudi, E. Razzazi-Fazeli, J. Bohm, and J. Zentek In Vitro Effects of Deoxynivalenol on Small Intestinal D-Glucose Uptake and Absorption of Deoxynivalenol Across the Isolated Jejunal Epithelium of Laying Hens Poult. Sci., January 1, 2007; 86(1): 15 - 20. [Abstract] [Full Text] [PDF]

				R. R. Pencek, Y. Koyama, D. B. Lacy, F. D. James, P. T. Fueger, K. Jabbour, P. E. Williams, and D. H. Wasserman Prior exercise enhances passive absorption of intraduodenal glucose J Appl Physiol, September 1, 2003; 95(3): 1132 - 1138. [Abstract] [Full Text] [PDF]