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© 2002 The American Society for Nutritional Sciences J. Nutr. 132:1115-1121, 2002

Biochemical and Molecular Actions of Nutrients

Trans-10,cis-12 Conjugated Linoleic Acid Suppresses the Desaturation of Linoleic and {alpha}-Linolenic Acids in HepG2 Cells1

Klaus Eder2, Nadine Slomma and Karin Becker

Institut für Ernährungswissenschaften, Martin-Luther-Universität Halle-Wittenberg, D-06108 Halle/Saale, Germany

2To whom correspondence should be addressed. E-mail: eder{at}landw.uni-halle.de

The objective of this study was to determine the effects of cis-9,trans-11 and trans-10,cis-12 CLA on the fatty acid desaturation in a human hepatoma cell line, HepG2. Therefore, experiments were conducted in which HepG2 cells were incubated with various concentrations of those fatty acids and the concentrations of fatty acids in various lipid fractions of HepG2 cells were determined. In the presence of linoleic acid as substrate, cells treated with 25 µmol/L of trans-10,cis-12 CLA had lower ratios of dihomo-{gamma}-linoleic acid to linoleic acid and of arachidonic acid to linoleic acid in phospholipids than control cells; with {alpha}-linolenic acid as substrate, they had a lower ratio of eicosapentaenoic acid to {alpha}-linolenic acid in phospholipids than control cells. Cells treated with cis-9,trans-11 CLA did not differ in these ratios from control cells. Cells treated with trans-10,cis-12 CLA had also a markedly lower ratio of mononunsaturated fatty acids (MUFA) to saturated fatty acids (SFA) in lipids than control cells; cells treated with cis-9,trans-11 CLA had a slightly lower MUFA:SFA ratio than control cells. These findings suggest that trans-10,cis-12 CLA suppresses {Delta}9-, {Delta}6- and {Delta}5-desaturation in HepG2 cells; cis-9,trans-11 CLA slightly reduces {Delta}9-desaturation but does not inhibit {Delta}6- and {Delta}5-desaturation. Moreover, HepG2 cells treated with 100 µmol/L of trans-10,cis-12 CLA released larger amounts of 6-keto-prostaglandin F1{alpha} and prostaglandin F2{alpha} than control cells. Treatment of cells with cis-9,trans-11 CLA did not alter the release of these eicosanoids compared with control cells. In conclusion, this study suggests that trans-10,cis-12 CLA has significant effects on the metabolism of essential fatty acids in HepG2 cells, whereas cis-9, trans-11 CLA does not have any effect in this respect.

KEY WORDS: • human hepatoma cells • conjugated linoleic acid • fatty acids • desaturation • eicosanoids




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