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,12
UMR 914, Physiologie de la Nutrition et du Comportement Alimentaire, Institut National Agronomique Paris-Grignon, Paris, France;
*
Laboratoire de Biologie Animale et Parasitaire, Faculté des Sciences Pharmaceutiques et Biologiques René Descartes, Paris, France;
Service de Coprologie Fonctionnelle, GH Pitié-Salpêtrière, Paris, France;
**
Service Commun d’Imagerie Cellulaire et Moléculaire, Faculté des Sciences Pharmaceutiques et Biologiques René Descartes, Paris, France;
Service d’Anatomie Pathologique, Hôpital Paul Brousse, Villejuif, France
2To whom correspondence should be addressed. E-mail: laurence.barbot{at}psl.ap-hop-paris.fr.
The ontogenetic development of PepT1, NBAT and EAAC1 along the vertical and horizontal axes of the rat small intestine was evaluated using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry. The proximodistal profiles of mRNA levels showed that PepT1 was evenly distributed, whereas NBAT had greater expression in the proximal part, and EAAC1 in the distal part. These regionalizations were the same from postnatal days 4 to 50. PepT1 and NBAT proteins were detected in the microvilli of enterocytes along the length of the villi. NBAT was also found in the cytoplasm. Surprisingly, EAAC1 was located exclusively in the microvilli of enterocytes in the crypt and the bases of the villi. These protein expression patterns were similar in all parts of the small intestine (proximal, median and distal), at all ages. We conclude that the expression of PepT1, NBAT or EAAC1 are differently regulated according to both the horizontal and vertical axes.
KEY WORDS: • ontogenesis • amino acid transporters • small intestine • rats
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