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Nutrient Metabolism

Trans-10, Cis-12 Conjugated Linoleic Acid Increases Fatty Acid Oxidation in 3T3-L1 Preadipocytes¹

Graduate Program in Nutrition, University of North Carolina at Greensboro, Greensboro, NC 27402 and ^* Department of Animal Science, North Carolina State University, Raleigh, NC 27695

²To whom correspondence should be addressed. E-mail: mkmcinto{at}uncg.edu.

The purpose of this study was to examine the effect of 0–50 µmol/L trans-10, cis-12 conjugated linoleic acid (CLA) and cis-9, trans-11 CLA isomers on lipid and glucose metabolism in cultures of differentiating 3T3-L1 preadipocytes. Specifically, we investigated the effects of 6 d of CLA treatment on the following: 1) ¹⁴C-glucose and ¹⁴C-oleic acid incorporation and esterification into lipid; 2) ¹⁴C-glucose and ¹⁴C-fatty acid oxidation; and 3) basal and isoproterenol-stimulated lipolysis. Trans-10, cis-12 CLA supplementation (25 and 50 µmol/L) increased both ¹⁴C-glucose and ¹⁴C-oleic acid incorporation into the cellular lipid fraction, which was primarily triglyceride (TG), compared with bovine serum albumin (BSA) controls. Although glucose oxidation (¹⁴C-glucose to ¹⁴C-CO₂) was unaffected by CLA supplementation, oleic acid oxidation (¹⁴C-oleic acid to ¹⁴C-CO₂) was increased by 55% in the presence of 50 µmol/L trans-10, cis-12 CLA compared with BSA controls. In contrast, 50 µmol/L linoleic acid (LA) and cis-9, trans-11 CLA-treated cultures had 50% lower CO₂ production from ¹⁴C-oleic acid compared with control cultures after 6 d of fatty acid exposure. Finally, 50 µmol/L trans-10, cis-12 CLA modestly increased basal, but not isoproterenol-stimulated lipolysis compared with control cultures. Thus, the TG-lowering actions of trans-10, cis-12 CLA in cultures of 3T3-L1 preadipocytes may be via increased fatty acid oxidation, which exceeded its stimulatory effects on glucose and oleic acid incorporation into lipid.

KEY WORDS: • conjugated linoleic acid • preadipocytes • glucose and fatty acid oxidation • glucose and fatty acid incorporation • lipolysis

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