![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
-Tocopherol Metabolism Is Abnormal in Scavenger Receptor Class B Type I (SR-BI)-Deficient Mice1
Departamentos de
*
Gastroenterología and
**
Nutrición, Diabetes y Metabolismo;
Facultad de Medicina and Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica, Santiago, Chile; and
Biology Department, Massachusetts Institute of Technology, Cambridge, MA 01239
2To whom correspondence should be addressed. E-mail: arigotti{at}med.puc.cl
Despite the physiologic importance of vitamin E, in particular its 
-tocopherol (-T) isoform, the molecular mechanisms involved in the cellular uptake of this antioxidant from plasma lipoproteins have not been well-defined. Recent studies have suggested that selective lipid uptake, rather than endocytosis, is important for -T delivery to cells. Here we show that the scavenger receptor class B type I (SR-BI), which mediates cellular selective cholesteryl ester uptake from lipoproteins, facilitates efficient transfer of -T from HDL to cultured cells. In SR-BI-deficient mutant mice, relative to wild-type control animals, there was a significant increase in plasma -T levels (1.1- to 1.4-fold higher) that was mostly due to the elevated -T content of their abnormally large plasma HDL-like particles. This increase in plasma -T in SR-BI knockout mice was accompanied by a significant decrease (65–80%) in the -T concentrations in bile and several tissues including ovary, testis, lung and brain. SR-BI deficiency did not alter the -T concentrations of the liver, spleen, kidney or white fat. These data show that SR-BI plays an important role in transferring -T from plasma lipoproteins to specific tissues. Also, in the case of the liver as was previously shown for SR-BI-dependent hepatic cholesterol transport, SR-BI-mediated uptake of -T was primarily coupled to biliary excretion rather than to tissue accumulation. Defective tissue uptake of lipoprotein -T in SR-BI-deficient mice may contribute to the reproductive and cardiovascular pathologies exhibited by these animals.
KEY WORDS: • lipoproteins • LDL receptor • vitamin E • mice • scavenger receptor class B type I
This article has been cited by other articles:
|
|
 |
|
  Y. Seino, T. Miki, H. Kiyonari, T. Abe, W. Fujimoto, K. Kimura, A. Takeuchi, Y. Takahashi, Y. Oiso, T. Iwanaga, et al. Isx Participates in the Maintenance of Vitamin A Metabolism by Regulation of {beta}-Carotene 15,15'-Monooxygenase (Bcmo1) Expression J. Biol. Chem., February 22, 2008; 283(8): 4905 - 4911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Borel, M. Moussa, E. Reboul, B. Lyan, C. Defoort, S. Vincent-Baudry, M. Maillot, M. Gastaldi, M. Darmon, H. Portugal, et al. Human Plasma Levels of Vitamin E and Carotenoids Are Associated with Genetic Polymorphisms in Genes Involved in Lipid Metabolism J. Nutr., December 1, 2007; 137(12): 2653 - 2659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Van Eck, M. Hoekstra, R. B. Hildebrand, Y. Yaong, D. Stengel, J. K. Kruijt, W. Sattler, U. J.F. Tietge, E. Ninio, T. J.C. Van Berkel, et al. Increased Oxidative Stress in Scavenger Receptor BI Knockout Mice With Dysfunctional HDL Arterioscler. Thromb. Vasc. Biol., November 1, 2007; 27(11): 2413 - 2419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Sheridan, N. Zhong, B. A. Carlson, C. M. Perella, D. L. Hatfield, and M. A. Beck Decreased Selenoprotein Expression Alters the Immune Response during Influenza Virus Infection in Mice J. Nutr., June 1, 2007; 137(6): 1466 - 1471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Abe, S. Ikeda, T. Uchida, K. Yamashita, and T. Ichikawa Triton WR1339, an Inhibitor of Lipoprotein Lipase, Decreases Vitamin E Concentration in Some Tissues of Rats by Inhibiting Its Transport to Liver J. Nutr., February 1, 2007; 137(2): 345 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Minehira-Castelli, S. W. Leonard, Q. M. Walker, M. G. Traber, and S. G. Young Absence of VLDL secretion does not affect {alpha}-tocopherol content in peripheral tissues J. Lipid Res., August 1, 2006; 47(8): 1733 - 1738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Reboul, A. Klein, F. Bietrix, B. Gleize, C. Malezet-Desmoulins, M. Schneider, A. Margotat, L. Lagrost, X. Collet, and P. Borel Scavenger Receptor Class B Type I (SR-BI) Is Involved in Vitamin E Transport across the Enterocyte J. Biol. Chem., February 24, 2006; 281(8): 4739 - 4745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Qian, S. Morley, K. Wilson, P. Nava, J. Atkinson, and D. Manor Intracellular trafficking of vitamin E in hepatocytes: the role of tocopherol transfer protein J. Lipid Res., October 1, 2005; 46(10): 2072 - 2082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zaripheh and J. W. Erdman Jr. The Biodistribution of a Single Oral Dose of [14C]-Lycopene in Rats Prefed Either a Control or Lycopene-Enriched Diet J. Nutr., September 1, 2005; 135(9): 2212 - 2218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. LODGE, W. L. HALL, Y. M. JEANES, and A. R. PROTEGGENTE Physiological Factors Influencing Vitamin E Biokinetics Ann. N.Y. Acad. Sci., December 1, 2004; 1031(1): 60 - 73. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Van Eck, I. S. T. Bos, R. B. Hildebrand, B. T. Van Rij, and T. J.C. Van Berkel Dual Role for Scavenger Receptor Class B, Type I on Bone Marrow-Derived Cells in Atherosclerotic Lesion Development Am. J. Pathol., September 1, 2004; 165(3): 785 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. F. Nieland, A. Chroni, M. L. Fitzgerald, Z. Maliga, V. I. Zannis, T. Kirchhausen, and M. Krieger Cross-inhibition of SR-BI- and ABCA1-mediated cholesterol transport by the small molecules BLT-4 and glyburide J. Lipid Res., July 1, 2004; 45(7): 1256 - 1265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Trigatti, M. Krieger, and A. Rigotti Influence of the HDL Receptor SR-BI on Lipoprotein Metabolism and Atherosclerosis Arterioscler. Thromb. Vasc. Biol., October 1, 2003; 23(10): 1732 - 1738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. L. Francone SR-BI: A New Player in an Old Game Arterioscler. Thromb. Vasc. Biol., September 1, 2003; 23(9): 1486 - 1487. [Full Text] [PDF]
|
|
|
|
|
|
M. Van Eck, J. Twisk, M. Hoekstra, B. T. Van Rij, C. A. C. Van der Lans, I. S. T. Bos, J. K. Kruijt, F. Kuipers, and T. J. C. Van Berkel Differential Effects of Scavenger Receptor BI Deficiency on Lipid Metabolism in Cells of the Arterial Wall and in the Liver J. Biol. Chem., June 20, 2003; 278(26): 23699 - 23705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rigotti, H. E. Miettinen, and M. Krieger The Role of the High-Density Lipoprotein Receptor SR-BI in the Lipid Metabolism of Endocrine and Other Tissues Endocr. Rev., June 1, 2003; 24(3): 357 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Panzenboeck, Z. Balazs, A. Sovic, A. Hrzenjak, S. Levak-Frank, A. Wintersperger, E. Malle, and W. Sattler ABCA1 and Scavenger Receptor Class B, Type I, Are Modulators of Reverse Sterol Transport at an in Vitro Blood-Brain Barrier Constituted of Porcine Brain Capillary Endothelial Cells J. Biol. Chem., November 1, 2002; 277(45): 42781 - 42789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kiefer, E. Sumser, M. F. Wernet, and J. von Lintig A class B scavenger receptor mediates the cellular uptake of carotenoids in Drosophila PNAS, August 6, 2002; 99(16): 10581 - 10586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Goti, Z. Balazs, U. Panzenboeck, A. Hrzenjak, H. Reicher, E. Wagner, R. Zechner, E. Malle, and W. Sattler Effects of Lipoprotein Lipase on Uptake and Transcytosis of Low Density Lipoprotein (LDL) and LDL-associated alpha -Tocopherol in a Porcine in Vitro Blood-Brain Barrier Model J. Biol. Chem., August 2, 2002; 277(32): 28537 - 28544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li, H.-Y. Kan, S. Lavrentiadou, M. Krieger, and V. Zannis Reconstituted Discoidal ApoE-Phospholipid Particles Are Ligands for the Scavenger Receptor BI. THE AMINO-TERMINAL 1-165 DOMAIN OF ApoE SUFFICES FOR RECEPTOR BINDING J. Biol. Chem., June 7, 2002; 277(24): 21149 - 21157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Liu, M. Krieger, H.-Y. Kan, and V. I. Zannis The Effects of Mutations in Helices 4 and 6 of ApoA-I on Scavenger Receptor Class B Type I (SR-BI)-mediated Cholesterol Efflux Suggest That Formation of a Productive Complex between Reconstituted High Density Lipoprotein and SR-BI Is Required for Efficient Lipid Transport J. Biol. Chem., June 7, 2002; 277(24): 21576 - 21584. [Abstract] [Full Text] [PDF]
|
|
