QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Panserat, S. Articles by Kaushik, S. Search for Related Content PubMed PubMed Citation Articles by Panserat, S. Articles by Kaushik, S.

High Dietary Lipids Induce Liver Glucose-6-Phosphatase Expression in Rainbow Trout (Oncorhynchus mykiss)

Laboratory of Fish Nutrition, INRA-IFREMER, 64310 St-Pée-sur-Nivelle, France

¹To whom correspondence should be addressed. E-mail: panserat{at}st-pee.inra.fr.

To contribute to the understanding of the mechanisms involved in poor metabolic utilization of dietary carbohydrates by rainbow trout (Oncorhynchus mykiss), we explored in this study the effects of dietary lipids on the regulation of two hepatic key enzymes, i.e., glucokinase (GK, first enzyme of the glycolytic pathway) and glucose-6-phosphatase (G6Pase, last enzyme of the gluconeogenesis). Two groups of juvenile trout were pair-fed for 8 wk either a low (10%) or a high (25%) level of dietary lipids supplied as fish oil; the pair-feeding technique was adopted to vary fat intake while keeping the protein and carbohydrate intakes more or less constant. Fish fed the high level of dietary lipids had inefficient control of glycemia compared with fish fed the low level of lipids. Levels of dietary lipids did not affect GK activity even though there was a small increase of GK mRNA level at 3 h after feeding high levels of lipids. By contrast, the high level of dietary lipids significantly increased G6Pase mRNA expression at 3, 6 and 12 h and enzyme activity at 6 h after food consumption. Thus, these data suggest that poor dietary carbohydrate utilization in rainbow trout may be related at least in part to increased hepatic glucose production under conditions of high dietary fat intake.

KEY WORDS: • dietary lipids • fish • hepatic glucose metabolism • glucokinase • glucose-6-phosphatase

This article has been cited by other articles:

				S. Morais, A. Knoll-Gellida, M. Andre, C. Barthe, and P. J. Babin Conserved expression of alternative splicing variants of peroxisomal acyl-CoA oxidase 1 in vertebrates and developmental and nutritional regulation in fish Physiol Genomics, February 12, 2007; 28(3): 239 - 252. [Abstract] [Full Text] [PDF]

				S. Kirchner, S. Kaushik, and S. Panserat Low Protein Intake Is Associated with Reduced Hepatic Gluconeogenic Enzyme Expression in Rainbow Trout (Oncorhynchus mykiss) J. Nutr., August 1, 2003; 133(8): 2561 - 2564. [Abstract] [Full Text] [PDF]