![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Roche Vitamins, Human Nutrition and Health, Carotenoid Group, CH-4070 Basel, Switzerland * INRA, Unité des Maladies Métaboliques, Equipe Vitamines, 63009 Clermont-Ferrand, France
1To whom correspondence should be addressed. E-mail: adrian.wyss{at}roche.com.
ß,ß-Carotene 15,15'-monooxygenase (formerly termed ß,ß-carotene 15,15'-dioxygenase, EC 1.13.11.21) catalyzes the conversion of provitamin A carotenoids to retinal in vertebrate tissues. In the present study, we investigated whether preformed vitamin A or ß-carotene and its direct metabolites can regulate the enzyme activity in vivo. We found dose-dependent decreases in intestinal ß,ß-carotene monooxygenase activity after oral administration to rats of retinyl acetate (up to -79%), ß-carotene (up to -79%), apo-8'-carotenal (up to -56%), all-trans retinoic acid (up to -88%), and 9-cis retinoic acid (up to -67%). Liver ß,ß-carotene 15,15'-monooxygenase (ßCMOOX) activity was not affected. Apo-12'carotenal and the retinoic acid receptor (RAR) 
antagonist Ro 41-5253 significantly increased the intestinal enzyme activity by 55 and 94%, respectively. When ß-carotene was administered to rats pretreated with the two cytochrome P450 (CYP) inducers, pentobarbital and naphthoflavone, the intestinal ßCMOOX activity increased by 39%. In a transcriptional study in chickens, treatment with retinoic acid resulted in low expression of the intestinal ßCMOOX. Our data suggest that retinoids and carotenoids might regulate ßCMOOX expression by a transcriptional feedback mechanism via interaction with members of the RAR family.
KEY WORDS: • ß-carotene cleavage • retinoic acid regulation • RAR • rats • chickens
This article has been cited by other articles:
|
|
 |
|
  W. C. Leung, S. Hessel, C. Meplan, J. Flint, V. Oberhauser, F. Tourniaire, J. E. Hesketh, J. von Lintig, and G. Lietz Two common single nucleotide polymorphisms in the gene encoding {beta}-carotene 15,15'-monoxygenase alter {beta}-carotene metabolism in female volunteers FASEB J, April 1, 2009; 23(4): 1041 - 1053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Seino, T. Miki, H. Kiyonari, T. Abe, W. Fujimoto, K. Kimura, A. Takeuchi, Y. Takahashi, Y. Oiso, T. Iwanaga, et al. Isx Participates in the Maintenance of Vitamin A Metabolism by Regulation of {beta}-Carotene 15,15'-Monooxygenase (Bcmo1) Expression J. Biol. Chem., February 22, 2008; 283(8): 4905 - 4911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Zaripheh, T. Y. Nara, M. T. Nakamura, and J. W. Erdman Jr. Dietary Lycopene Downregulates Carotenoid 15,15'-Monooxygenase and PPAR-{gamma} in Selected Rat Tissues J. Nutr., April 1, 2006; 136(4): 932 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Chichili, D. Nohr, M. Schaffer, J. von Lintig, and H. K. Biesalski {beta}-Carotene Conversion into Vitamin A in Human Retinal Pigment Epithelial Cells Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3562 - 3569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wyss Carotene Oxygenases: A New Family of Double Bond Cleavage Enzymes J. Nutr., January 1, 2004; 134(1): 246S - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Lemke, S. R. Dueker, J. R. Follett, Y. Lin, C. Carkeet, B. A. Buchholz, J. S. Vogel, and A. J. Clifford Absorption and retinol equivalence of {beta}-carotene in humans is influenced by dietary vitamin A intake J. Lipid Res., August 1, 2003; 44(8): 1591 - 1600. [Abstract] [Full Text] [PDF]
|
|
