QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nieto, N. Articles by Gil, A. Search for Related Content PubMed PubMed Citation Articles by Nieto, N. Articles by Gil, A.

Dietary Polyunsaturated Fatty Acids Improve Histological and Biochemical Alterations in Rats with Experimental Ulcerative Colitis¹

Natalia Nieto^*, María Isabel Torres, Antonio Ríos^* and Angel Gil^*2

Departments of ^* Biochemistry and Molecular Biology and Cell Biology, University of Granada, 18071 Granada, Spain

²To whom correspondence should be addressed. E-mail: agil{at}goliat.ugr.es

The aim of the present study was to determine whether dietary intake of monounsaturated (MUFA) and/or polyunsaturated fatty acids (PUFA) of the (n- 3) and (n-6) series could improve intestinal damage and reduce inflammation in experimental ulcerative colitis (UC). Rats were treated with 80 mg/kg body of 2,4,6-trinitrobenzenesulfonic acid and fed for 1 or 2 wk diets enriched in olive oil (OO), fish oil (FO), or purified pig brain phospholipids (BPL), as sources of monounsaturated and PUFA of the (n-3) and (n-3) + (n-6) series. Evaluation of macroscopic and microscopic colonic damage was assessed. Ultrastructural and histologic changes were analyzed as well as plasma and colonic mucosa fatty acid profiles and some biochemical markers of injury and inflammation [alkaline phosphatase (AP), mieloperoxidase (MPO), prostaglandin E₂ (PGE₂) and leukotriene B₄]. Fatty acid profiles of both plasma and mucosa mostly reflected the dietary fatty acid composition. Plasma MUFA proportions were higher in UC animals fed the OO diet compared with FO or BPL groups 1 and 2 wk and (n-3) long chain PUFA (LC-PUFA) were higher in the FO than in the OO and BPL groups. At 1 wk, UC led to lower MUFA mucosa levels and (n-3)LC-PUFA were higher in the FO group compared with the OO and BPL groups. Rats with UC fed FO at 1 wk showed significantly less macroscopic and microscopic colonic damage. They also have lower AP and MPO activities and PGE₂ levels compared with the OO and BPL groups and showed enhanced histological repair, less necrotic areas within the mucosa, and more goblet cells with mature mucin granules. These results suggest that the use of balanced diets containing (n-3) LC-PUFA could ameliorate the inflammation and mucosal damage in UC.

KEY WORDS: • trinitrobenzenesulfonic acid • inflammatory bowel disease • ulcerative colitis • fatty acids

This article has been cited by other articles:

				R. A. Siddiqui, K. A. Harvey, G. P. Zaloga, and W. Stillwell Modulation of Lipid Rafts by {Omega}-3 Fatty Acids in Inflammation and Cancer: Implications for Use of Lipids During Nutrition Support Nutr Clin Pract, February 1, 2007; 22(1): 74 - 88. [Abstract] [Full Text] [PDF]

				R. S. Chapkin, L. A. Davidson, L. Ly, B. R. Weeks, J. R. Lupton, and D. N. McMurray Immunomodulatory Effects of (n-3) Fatty Acids: Putative Link to Inflammation and Colon Cancer J. Nutr., January 1, 2007; 137(1): 200S - 204S. [Abstract] [Full Text] [PDF]

				D. Camuesco, J. Galvez, A. Nieto, M. Comalada, M. E. Rodriguez-Cabezas, A. Concha, J. Xaus, and A. Zarzuelo Dietary Olive Oil Supplemented with Fish Oil, Rich in EPA and DHA (n-3) Polyunsaturated Fatty Acids, Attenuates Colonic Inflammation in Rats with DSS-Induced Colitis J. Nutr., April 1, 2005; 135(4): 687 - 694. [Abstract] [Full Text] [PDF]

				G. S. Patten, M. Y. Abeywardena, E. J. McMurchie, and A. Jahangiri Dietary Fish Oil Increases Acetylcholine- and Eicosanoid-Induced Contractility of Isolated Rat Ileum J. Nutr., September 1, 2002; 132(9): 2506 - 2513. [Abstract] [Full Text] [PDF]