![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
UPRES Lipids & Nutrition, Faculty of Life Sciences, Université de Bourgogne, Dijon 21000, France
2To whom correspondence should be addressed. E-mail: naim.khan{at}u-bourgogne.fr.
After 10 wk of feeding an experimental diet enriched with (n-3) polyunsaturated fatty acids (PUFA), i.e., eicosapentaenoic acid [EPA, 20:5(n-3)] and [DHA, 22:6(n-3)] (EPAX), blood pressure in spontaneously hypertensive rats (SHR), but not in normotensive Wistar-Kyoto (WKY) rats was reduced relative to rats fed an unsupplemented control diet. Concavalin A–stimulated T-cell proliferation was diminished in both strains of rats fed the PUFA/EPAX diet. The experimental diet lowered secretion of interleukin-2 in SHR, but not in WKY rats compared with rats fed the control diet. To determine whether there was a defect in calcium homeostasis in T cells during hypertension, we employed the following agents: caffeine, which recruits calcium from the cytosolic Ca2+-induced Ca2+-release pool; ionomycin, which at low concentrations opens calcium channels; and thapsigargin (TG), which mobilizes [Ca2+]i from the endoplasmic reticulum (ER) pool. Caffeine-induced increases in [Ca2+]i were not modified by the PUFA/EPAX diet. The ionomycin-induced increases in [Ca2+]i in T cells from SHR were greater than in those from WKY rats; consumption of the PUFA/EPAX diet did not modify Ca2+ influx in cells of either strain. The TG-induced increases in [Ca2+]i in T cells from SHR were greater than those in cells from WKY rats. Interestingly, consumption of the experimental diet reduced TG-evoked increases in [Ca2+]i in T cells from SHR and increased those in T cells from WKY rats, indicating that the PUFA/EPAX diet could reverse the calcium mobilization from the ER pool in T cells. These results suggest that (n-3) PUFA exert antihypertensive effects and modulate T-cell calcium signaling during hypertension in rats.
KEY WORDS: • (n-3) fatty acids • hypertension • calcium • immunomodulation • rats
This article has been cited by other articles:
|
|
 |
|
  H. Truong, J. R DiBello, E. Ruiz-Narvaez, P. Kraft, H. Campos, and A. Baylin Does genetic variation in the {Delta}6-desaturase promoter modify the association between {alpha}-linolenic acid and the prevalence of metabolic syndrome? Am. J. Clinical Nutrition, March 1, 2009; 89(3): 920 - 925. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. N. Kolar, R. Barhoumi, J. R. Lupton, and R. S. Chapkin Docosahexaenoic Acid and Butyrate Synergistically Induce Colonocyte Apoptosis by Enhancing Mitochondrial Ca2+ Accumulation Cancer Res., June 1, 2007; 67(11): 5561 - 5568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Petursdottir and I. Hardardottir Dietary Fish Oil Increases the Number of Splenic Macrophages Secreting TNF-{alpha} and IL-10 But Decreases the Secretion of These Cytokines by Splenic T Cells from Mice J. Nutr., March 1, 2007; 137(3): 665 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Mies, V. Shlyonsky, A. Goolaerts, and S. Sariban-Sohraby Modulation of epithelial Na+ channel activity by long-chain n-3 fatty acids Am J Physiol Renal Physiol, October 1, 2004; 287(4): F850 - F855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. deGraffenried, W. E. Friedrichs, L. Fulcher, G. Fernandes, J. M. Silva, J.-M. Peralba, and M. Hidalgo Eicosapentaenoic acid restores tamoxifen sensitivity in breast cancer cells with high Akt activity Ann. Onc., July 1, 2003; 14(7): 1051 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Patten, M. Y. Abeywardena, E. J. McMurchie, and A. Jahangiri Dietary Fish Oil Increases Acetylcholine- and Eicosanoid-Induced Contractility of Isolated Rat Ileum J. Nutr., September 1, 2002; 132(9): 2506 - 2513. [Abstract] [Full Text] [PDF]
|
|
