QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Matsuzaki, J. Articles by Nakano, Y. Search for Related Content PubMed PubMed Citation Articles by Matsuzaki, J. Articles by Nakano, Y.

---

Articles

Inflammatory Responses to Lipopolysaccharide Are Suppressed in 40% Energy-Restricted Mice

Junko Matsuzaki^*, Mitsuru Kuwamura, Ryoichi Yamaji^*, Hiroshi Inui and Yoshihisa Nakano^*

Departments of ^* Applied Biological Chemistry and Veterinary Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan

¹To whom correspondence should be addressed. E-mail: inui{at}biochem.osakafu-u.ac.jp

To elucidate the suppressive effects of energy restriction on the inflammatory responses to lipopolysaccharide (LPS), mice were divided into a control group (fed 5.0 g diet/d; 71 kJ/d) and a 40% energy-restricted group (fed 3.0 g diet/d; 43 kJ/d) at 8-wk of age. Four weeks later, 25 µg of LPS was intraperitoneally injected. After the LPS injection, interleukin-1ß, interleukin-6 and tumor necrosis factor- were elevated in serums in the 40% energy-restricted mice and in the controls, but the extent of the elevation was significantly lower in the restricted group. The LPS-induced expression of inducible nitric oxide synthase in the liver was significantly suppressed by the energy restriction. In addition, the LPS-induced elevations of serum aspartate and alanine aminotransferase activities, which are indexes of hepatic injury, were also significantly attenuated in the restricted group. Moreover, the extent of LPS-induced alterations in hepatic structure was less in the restricted mice than in controls. Serum corticosterone level in the restricted mice was higher than that in the controls before LPS treatment (P < 0.05). Furthermore, after LPS injection, the significantly higher level of corticosterone was maintained in the restricted mice, although the LPS treatment significantly enhanced the level even in the control group. These results suggest that the extreme inflammatory responses to endotoxin are prevented in the 40% energy-restricted mice, and corticosterone participates in the preventive effects.

KEY WORDS: • energy-restricted mice • lipopolysaccharide • proinflammatory cytokines • inducible nitric oxide synthase • glucocorticoids

This article has been cited by other articles:

				L. Piccio, J. L. Stark, and A. H. Cross Chronic calorie restriction attenuates experimental autoimmune encephalomyelitis J. Leukoc. Biol., October 1, 2008; 84(4): 940 - 948. [Abstract] [Full Text] [PDF]

				K. A. Schubert, L. M. Vaanholt, F. Stavasius, G. E. Demas, S. Daan, and G. H. Visser Female mice respond differently to costly foraging versus food restriction J. Exp. Biol., July 15, 2008; 211(14): 2214 - 2223. [Abstract] [Full Text] [PDF]

				D. B. Rozema, D. L. Lewis, D. H. Wakefield, S. C. Wong, J. J. Klein, P. L. Roesch, S. L. Bertin, T. W. Reppen, Q. Chu, A. V. Blokhin, et al. Dynamic PolyConjugates for targeted in vivo delivery of siRNA to hepatocytes PNAS, August 7, 2007; 104(32): 12982 - 12987. [Abstract] [Full Text] [PDF]

				L. Fontana and S. Klein Aging, Adiposity, and Calorie Restriction JAMA, March 7, 2007; 297(9): 986 - 994. [Abstract] [Full Text] [PDF]

				L. Fontana Excessive Adiposity, Calorie Restriction, and Aging JAMA, April 5, 2006; 295(13): 1577 - 1578. [Full Text] [PDF]

				K. N. Jeejeebhoy Permissive Underfeeding of the Critically Ill Patient Nutr Clin Pract, October 1, 2004; 19(5): 477 - 480. [Abstract] [Full Text] [PDF]

				L. Fontana, T. E. Meyer, S. Klein, and J. O. Holloszy Long-term calorie restriction is highly effective in reducing the risk for atherosclerosis in humans PNAS, April 27, 2004; 101(17): 6659 - 6663. [Abstract] [Full Text] [PDF]