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Articles

Functional Fragments of Ingested Lactoferrin Are Resistant to Proteolytic Degradation in the Gastrointestinal Tract of Adult Rats¹

Nutritional Science Laboratory and ^* Biochemical Research Laboratory, Morinaga Milk Industry Company, Zama, Kanagawa 228-8583, Japan

²To whom correspondence should be addressed. E-mail: jcb00322{at}nifty.ne.jp.

Pharmaceutical and food-related applications of lactoferrin, an 80-kDa iron-binding glycoprotein found predominantly in milk, have attracted interest lately, but the process of digestion of lactoferrin has been poorly characterized. The digestive fate of bovine lactoferrin in adult rats after oral administration of a single dose and after dietary supplementation was studied by ¹²⁵I-labeling and by surface-enhanced laser desorption/ionization (SELDI) affinity mass spectrometry. The latter method was designed to detect multiple forms of degraded lactoferrin as simple molecular ion peaks corresponding to one of the core regions of lactoferrin, namely, the lactoferricin region (Phe17-Ala42). Radioactive fragments with molecular masses of 42, 36, 33 and 29 kDa were observed at 20, 60 and 180 min postingestion in the contents of the lower small intestine. Rats were given free access to milk enriched with lactoferrin at 482 µmol/L (40 mg/mL). The concentrations of lactoferrin fragments in the contents of the stomach, small intestine and lower small intestine as determined by SELDI affinity mass spectrometry were 200, 20 and 1 µmol/L, respectively. These data indicate that functional fragments of LF such as fragments containing glycosaminoglycan-binding site(s), as well as large fragments with a mass >20 kDa, indeed survive proteolytic degradation in the small intestine of adult rats.

KEY WORDS: • lactoferrin • lactoferricin • digestion • SELDI affinity mass spectrometry • rats

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