QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kuvibidila, S. R. Articles by Baliga, B. S. Search for Related Content PubMed PubMed Citation Articles by Kuvibidila, S. R. Articles by Baliga, B. S.

---

Articles

Iron Deficiency Alters the Progression of Mitogen-Treated Murine Splenic Lymphocytes through the Cell Cycle¹

Solo R. Kuvibidila^*,2, Connie Porretta and B. Surendra Baliga^**

^* Department of Pediatrics, Divisions of Hematology/Oncology and the Department of Pulmonary Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112 and the ^** Department of Pediatrics, University of South Alabama College of Medicine, Mobile, AL 36617

²To whom correspondence should be addressed. E-mail: skuvib{at}lsuhsc.edu

The influence of iron deficiency on the progression of mitogen-treated splenic lymphocytes through the cell cycle was studied in 16 control, 16 pair-fed, 15 iron-deficient (ID) and 16 ID mice that were repleted for up to 3 d (R3). The test and control diets differed only in iron concentrations (0.09 vs. 0.9 mmol/kg). When mice were killed (68 d of feeding), the hemoglobin concentration and liver iron stores of ID and R3 mice were <50% those of control mice (P < 0.05). Iron deficiency did not reduce the percentage of CD3⁺ cells, but decreased CD3⁺ cells/mg spleen (P < 0.05). In concanavalin A-treated and nonactivated cultures, there were no significant differences among groups in the percentages of cells in resting phase of the cell cycle (G0) to cell cycle initiation phase (G1), DNA synthesis phase (S) and exit from the S phase (G2) to mitosis phase (M) phases. In anti-CD3 and anti-CD3/anti-CD28-treated cultures, higher percentages of lymphocytes from ID and R3 mice than those from control and pair-fed mice were in the G0–G1 phase (P < 0.05). Conversely, lower percentages of activated cells from ID and R mice than those from control and pair-fed mice were in S and G2–M phases (P < 0.05). Incubation of lymphocytes with mitogens decreased the percentages of cells in G0–G1 phase from 90% to 80% in control and pair-fed but not in ID and R3 mice (P < 0.05). In activated cells, indices of iron status negatively correlated with the percentages of cells in G0–G1 (r = -0.306 to -0.597) but positively with those in S (r = 0.166–0.511) and G2–M phases (r = 0.265–0.59; P < 0.05). Data suggest that altered cell cycle progression likely contributes to impaired lymphocyte proliferation usually associated with iron deficiency.

KEY WORDS: • iron deficiency • lymphocyte proliferation • concanavalin A • cell cycle analysis • mice

This article has been cited by other articles:

				C. J. Field, I. R. Johnson, and P. D. Schley Nutrients and their role in host resistance to infection J. Leukoc. Biol., January 1, 2002; 71(1): 16 - 32. [Abstract] [Full Text] [PDF]