![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Unidad de Genética de la Nutrición of the Instituto de Investigaciones Biomédicas, UNAM and Instituto Nacional de Pediatría, México DF 04530
2To whom correspondence should be addressed. E-mail: velare{at}servidor.unam.mx
Biotin is the cofactor of carboxylases [pyruvate (PC), propionyl-CoA (PCC), 3-methyl crotonyl-CoA and acetyl-CoA], to which it is covalently bound by the action of holocarboxylase synthetase (HCS). We have studied whether biotin also regulates their expression, as it does other, nonrelated enzymes (e.g., glucokinase, phosphoenol pyruvate carboxykinase, guanylate cyclase). For this purpose, HCS, PC and PCC mRNAs were studied in biotin-deficient rat liver, kidney, muscle and brain of biotin-deficient rats. PC- and PCC-specific activities and protein masses were also measured. The 24-h time course of HCS mRNA in deficient rats was examined after biotin supplementation. HCS mRNA was significantly reduced during vitamin deficiency. It increased in deficient rats after biotin was injected, reaching control levels 24 h after administration. These changes seem to be the first known instance in mammals of an effect of a water-soluble vitamin on a mRNA functionally related to it. In contrast, the decreased activities of the carboxylases were associated with reductions in the amounts of their enzyme proteins except in brain. However, their mRNA levels were not affected. There are no reports on these types of vitamin affecting the mRNA or protein levels of their apoenzymes or their products. This work provides evidence for biotin being a modulator of the genetic expression of the enzymes involved in its function as a cofactor. As such, it may be a useful model for probing a similar role for other water-soluble vitamins.
KEY WORDS: • biotin • carboxylases • holocarboxylase synthetase • gene expression • rats
This article has been cited by other articles:
|
|
 |
|
  R. Takechi, A. Taniguchi, S. Ebara, T. Fukui, and T. Watanabe Biotin Deficiency Affects the Proliferation of Human Embryonic Palatal Mesenchymal Cells in Culture J. Nutr., April 1, 2008; 138(4): 680 - 684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ferreira and W. P. Weiss Effect of Biotin on Activity and Gene Expression of Biotin-Dependent Carboxylases in the Liver of Dairy Cows J Dairy Sci, March 1, 2007; 90(3): 1460 - 1466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. M. Sealey, S. L. Stratton, D. M. Mock, and D. K. Hansen Marginal Maternal Biotin Deficiency in CD-1 Mice Reduces Fetal Mass of Biotin-dependent Carboxylases J. Nutr., May 1, 2005; 135(5): 973 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. I. Vlasova, S. L. Stratton, A. M. Wells, N. I. Mock, and D. M. Mock Biotin Deficiency Reduces Expression of SLC19A3, a Potential Biotin Transporter, in Leukocytes from Human Blood J. Nutr., January 1, 2005; 135(1): 42 - 47. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Pacheco-Alvarez, R. S. Solorzano-Vargas, R. A. Gravel, R. Cervantes-Roldan, A. Velazquez, and A. Leon-Del-Rio Paradoxical Regulation of Biotin Utilization in Brain and Liver and Implications for Inherited Multiple Carboxylase Deficiency J. Biol. Chem., December 10, 2004; 279(50): 52312 - 52318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Rodriguez-Melendez, J. B. Griffin, and J. Zempleni Biotin Supplementation Increases Expression of the Cytochrome P450 1B1 Gene in Jurkat Cells, Increasing the Occurrence of Single-Stranded DNA Breaks J. Nutr., September 1, 2004; 134(9): 2222 - 2228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Rosendo, C. R. Staples, L. R. McDowell, R. McMahon, L. Badinga, F. G. Martin, J. F. Shearer, W. M. Seymour, and N. S. Wilkinson Effects of Biotin Supplementation on Peripartum Performance and Metabolites of Holstein Cows J Dairy Sci, August 1, 2004; 87(8): 2535 - 2545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Baez-Saldana and E. Ortega Biotin Deficiency Blocks Thymocyte Maturation, Accelerates Thymus Involution, and Decreases Nose-Rump Length in Mice J. Nutr., August 1, 2004; 134(8): 1970 - 1977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Griffin, R. Rodriguez-Melendez, and J. Zempleni The Nuclear Abundance of Transcription Factors Sp1 and Sp3 Depends on Biotin in Jurkat Cells J. Nutr., November 1, 2003; 133(11): 3409 - 3415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Rathman, R. K. Blanchard, L. Badinga, J. F. Gregory III, S. Eisenschenk, and R. J. McMahon Dietary Carbamazepine Administration Decreases Liver Pyruvate Carboxylase Activity and Biotinylation by Decreasing Protein and mRNA Expression in Rats J. Nutr., July 1, 2003; 133(7): 2119 - 2124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Camporeale and J. Zempleni Oxidative Folding of Interleukin-2 Is Impaired in Flavin-Deficient Jurkat Cells, Causing Intracellular Accumulation of Interleukin-2 and Increased Expression of Stress Response Genes J. Nutr., March 1, 2003; 133(3): 668 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wiedmann, J. D. Eudy, and J. Zempleni Biotin Supplementation Increases Expression of Genes Encoding Interferon-{gamma}, Interleukin-1{beta}, and 3-Methylcrotonyl-CoA Carboxylase, and Decreases Expression of the Gene Encoding Interleukin-4 in Human Peripheral Blood Mononuclear Cells J. Nutr., March 1, 2003; 133(3): 716 - 719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Stanley, D. M. Mock, J. B. Griffin, and J. Zempleni Biotin Uptake into Human Peripheral Blood Mononuclear Cells Increases Early in the Cell Cycle, Increasing Carboxylase Activities J. Nutr., July 1, 2002; 132(7): 1854 - 1859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Manthey, J. B. Griffin, and J. Zempleni Biotin Supply Affects Expression of Biotin Transporters, Biotinylation of Carboxylases and Metabolism of Interleukin-2 in Jurkat Cells J. Nutr., May 1, 2002; 132(5): 887 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Solorzano-Vargas, D. Pacheco-Alvarez, and A. Leon-Del-Rio Holocarboxylase synthetase is an obligate participant in biotin-mediated regulation of its own expression and of biotin-dependent carboxylases mRNA levels in human cells PNAS, April 16, 2002; 99(8): 5325 - 5330. [Abstract] [Full Text] [PDF]
|
|
