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,**2
*
Departments of Animal Sciences and
**
Veterinary Pathobiology and
Division of Nutritional Sciences, The University of Illinois at Urbana-Champaign, Urbana, IL 61801
2To whom correspondence should be addressed. E-mail: hgaskins{at}uiuc.edu.
A cultivation-independent approach, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), was used to characterize changes in fecal bacterial populations resulting from consumption of a low residue diet or oral administration of a broad-spectrum antibiotic. C57BL/6NHsd mice were weaned to either a standard nonpurified diet (LC-diet) or a low residue diet (LR-diet) and at 17 wk of age were randomly assigned to receive drinking water with or without 25 ppm cefoxitin for 14 d. On d 1, 2, 7 and 14, microbial DNA was extracted from feces, and the V3 region of the 16S rDNA gene was amplified by PCR and analyzed by DGGE. The diversity of fecal microbial populations, assessed using Shannon’s index (H'), which incorporates species richness (number of species, or in this case, PCR-DGGE bands) and evenness (the relative distribution of species), was not affected by cefoxitin. However, use of Sorenson’s pairwise similarity coefficient (Cs), an index that measures the species in common between different habitats, indicated that the species composition of fecal bacterial communities was altered by cefoxitin in mice fed either diet. Dietary effects on fecal microbial communities were more pronounced, with greater H' values (P < 0.05) in mice fed the LR-diet (1.9 ± 0.1) compared with the LC-diet (1.6 ± 0.1). The Cs values were also greater (P < 0.05) in fecal bacterial populations from mice fed the LR-diet (Cs = 69.8 ± 2.0%) compared with mice fed the LC-diet (Cs = 50.1 ± 3.8%), indicating greater homogeneity of fecal bacterial communities in mice fed the LR-diet. These results demonstrate the utility of cultivation-independent PCR-DGGE analysis combined with measurements of ecological diversity for monitoring diet- and antibiotic-induced alterations of the complex intestinal microbial ecosystem.
KEY WORDS: • antibiotic • PCR-DGGE • fiber • intestinal microbiota • microbial ecology
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