QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Liu, Z. Articles by Kato, N. Search for Related Content PubMed PubMed Citation Articles by Liu, Z. Articles by Kato, N.

---

Research Communication

A Buckwheat Protein Product Suppresses 1,2-Dimethylhydrazine–Induced Colon Carcinogenesis in Rats by Reducing Cell Proliferation

Zhihe Liu, Wakako Ishikawa, Xuxin Huang, Hiroyuki Tomotake^*, Jun Kayashita, Hiromitsu Watanabe^** and Norihisa Kato¹

Department of Applied Biochemistry, Hiroshima University, Higashi-Hiroshima 739-8528, Japan; ^* Iida Women’s Junior College, Nagano 395-0812, Japan; Gifu City Women’s College, Gifu 501-0192, Japan; and ^** Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-0037, Japan

¹To whom correspondence should be addressed. E-mail: nkato{at}hiroshima-u.ac.jp.

This study was conducted to examine the effect of consumption of buckwheat protein product (BWP) on 1,2-dimethylhydrazine (DMH)-induced colon tumor in rats. Male growing Sprague-Dawley rats were fed diets containing either casein or BWP (net protein level, 200 g/kg; n = 20/group) for 124 d. The rats were gavaged weekly with DMH (20 mg/kg body) for the first 8 wk. Food intake and growth were unaffected by dietary manipulation. Dietary BWP caused a 47% reduction in the incidence of colonic adenocarcinoma (P < 0.05), but did not affect the incidence of colonic adenomas. BWP intake tended to reduce the number of colon adenocarcinomas (P = 0.16). Consumption of BWP significantly reduced cell proliferation and expression of c-myc and c-fos proteins in colonic epithelium. The results suggest that dietary BWP has a protective effect against DMH-induced colon carcinogenesis in rats by reducing cell proliferation.

KEY WORDS: • Buckwheat protein • 1–2-dimethylhydrazine • colon carcinogenesis • cell proliferation • rats