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Department of Animal Science, Cornell University, Ithaca, NY 14853
2To whom correspondence should be addressed. E-mail: deb6{at}cornell.edu.
Conjugated linoleic acid (CLA) supplements containing a variety of
isomers reduce milk fat yield. We have recently identified
trans-10, cis-12 CLA as the isomer
responsible for inhibiting milk fat synthesis in dairy cows. Our
objectives were to determine milk fat yield and fatty acid composition
responses to different doses of trans-10,
cis-12 CLA. Multiparous Holstein cows (n
= 4) were used in a 4 x 4 Latin square design. Treatments
consisted of a 5-d abomasal infusion of four doses of
trans-10, cis-12 CLA, i.e., 0.0, 3.5, 7.0
and 14.0 g/d. Milk fat yield was decreased 25, 33, and 50%, and milk
fat concentration was reduced 24, 37 and 46% when cows received 3.5,
7.0 and 14.0 g/d of trans-10, cis-12 CLA,
respectively. Feed intake, milk yield, and milk protein content and
yield were unaffected by treatment. Milk fatty acid composition
revealed that de novo synthesized fatty acids (short and medium chain)
were extensively reduced when cows received the two highest doses, but
at the low dose (3.5 g/d), decreases in de novo synthesized fatty acids
and preformed fatty acids were similar. Changes in milk fatty acid
composition also demonstrated that 
9-desaturase activity
was inhibited at the two high doses of trans-10,
cis-12 CLA, but was unaffected by the low dose. Results
indicate minimal quantities of trans-10,
cis-12 CLA (0.016% of dietary dry matter)
markedly inhibited milk fat synthesis (25% reduction) and that a
curvilinear reduction in milk fat yield occurred with increasing
quantities of trans-10, cis-12 CLA.
KEY WORDS: • conjugated linoleic acid • milk fat • lactation • fatty acids • cows
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