Journal of Nutrition Animal Diets/Enrichment Products...

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Manuel y Keenoy, B.
Right arrow Articles by De Leeuw, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Manuel y Keenoy, B.
Right arrow Articles by De Leeuw, I.
(Journal of Nutrition. 2001;131:1723-1730.)
© 2001 The American Society for Nutritional Sciences

Articles

Long-Term Pharmacologic Doses of Vitamin E Only Moderately Affect the Erythrocytes of Patients with Type 1 Diabetes Mellitus1

Begoña Manuel y Keenoy2, Honglei Shen, Wendy Engelen, Jan Vertommen, Guido Van Dessel*, Albert Lagrou* and Ivo De Leeuw

Laboratory of Endocrinology and * Laboratory of Human and Pathological Biochemistry, University of Antwerp, B-2610 Wilrijk-Antwerp, Belgium

2To whom correspondence should be addressed. E-mail: begona{at}uia.ua.ac.be.

In erythrocytes from diabetic patients, increased membrane lipid peroxidation might lead to abnormalities in composition and function. To study this relationship, we investigated the effects of a moderate pharmacologic dose of vitamin E for 1 y on erythrocyte membrane peroxidation in vitro and on its fatty acid composition, antioxidant capacity and rheological function. In a random and double-blind manner, type 1 diabetic patients (n = 44) were assigned to the following two groups: Group S received 250 IU (168 mg) d-{alpha} tocopherol 3 times daily for 1 y. Group P received placebo for 6 mo followed by d-{alpha}-tocopherol for an additional 6 mo. Variables were monitored every 3 mo. After 3 mo of supplementation, serum vitamin E doubled (P < 0.0005), thiobarbituric acid reactive substances in erythrocyte membranes incubated with tert-butyl hydroperoxide decreased by 25% (P = 0.006) and the lagtime of fluorescence increased from 28 ± 16 to 41 ± 28 min (P = 0.028). Patients who did not respond to supplementation (13 of 44) had lower serum lipids (P = 0.017) and body mass index (P = 0.024). We did not detect any significant effects of vitamin E supplementation on membrane lipid composition, antioxidant capacity or blood viscosity. Continuing supplementation for up to 1 y did not further affect serum vitamin E or membrane peroxidation. Stopping supplementation was followed by a return to inclusion values. These results show that the decrease in erythrocyte membrane peroxidation after vitamin E supplementation is moderate, saturable, reversible, restricted to some individuals and has no detectable effect on erythrocyte composition and function.

KEY WORDS: • vitamin E • type 1 diabetes mellitus • erythrocytes • lipid peroxidation • membranes • viscosity • humans






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]