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Dietary Fructooligosaccharides Modify Intestinal Bioavailability of a Single Dose of Genistein and Daidzein and Affect Their Urinary Excretion and Kinetics in Blood of Rats

Mariko Uehara¹, Atsutane Ohta^*, Kensuke Sakai^*, Kazuharu Suzuki, Shaw Watanabe and Herman Adlercreutz

Department of Nutritional Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan; ^* Bioscience Laboratories, Meiji Seika Kaisha, Limited, Sakado-city, Saitama 350-0289, Japan; and Institute for Preventive Medicine, Nutrition and Cancer, Folkhälsan Research Center, Division of Clinical Chemistry, University of Helsinki, PB 60 FIN-00014, Helsinki, Finland

¹To whom correspondence should be addressed. E-mail: mariu{at}attglobal.net.

The influence of dietary fructooligosaccharides (FOS) on bioavailability of genistein and daidzein in rats was estimated by measuring their concentrations in plasma collected from three different veins and in urine after a single intragastric administration of isoflavone conjugates. Sprague-Dawley male rats (6 wk old, n = 22) were fed a purified control (AIN-93G) diet or a FOS diet (AIN-93G + 5% FOS) for 7 d. A single dose of soy isoflavone conjugates, i.e., 8.5 mg as genistein and 33 mg as daidzein/kg body, was administered via a stomach tube at d 5. Blood samples were collected after administration via catheters in the portal and central veins and by puncture of the tail vein. The isoflavones in plasma and urine were analyzed by time-resolved fluoroimmunoassay. The genistein concentration in the portal blood increased rapidly, reaching a peak of 3.5 µmol/L in both groups at 1 h after administration. The concentrations in the central and tail venous blood were approximately half of those in the portal blood. In the FOS-fed group, both genistein and daidzein remained detectable at 24 and 48 h in the tail venous plasma. The urinary excretion of both isoflavones in the 24- to 48-h period after administration was significantly higher in the FOS-fed group than in the control group. The difference between the portal and central veins indicated hepatic uptake, probably leading to conjugation of aglycones and excretion into bile. FOS modified the absorption and enterohepatic recirculation of isoflavones.

KEY WORDS: • fructooligosaccharides • genistein • daidzein • rats • time-resolved fluoroimmunoassay

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