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Research Communication

The Proportion of CD45RA⁺CD62L⁺ (Quiescent-Phenotype) T Cells within the CD8⁺ Subset Increases in Advanced Weight Loss in the Protein- or Energy-Deficient Weanling Mouse¹

Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1

²To whom correspondence should be addressed. E-mail: wwoodwar{at}uoguelph.ca

Male and female C57BL/6J mice, initially 19 d old, had free access to a complete purified diet, were fed this diet in restricted daily quantities, or had free access to a low-protein diet. Three separate studies were conducted with feeding periods of 14, 9 or 6 d (n = 7–8 per dietary group and feeding period; 6 d: restricted intake and age-matched controls only). A zero-time control group (19 d old) was included in each study. Malnourished mice lost 2% of initial body weight daily. Naïve-phenotype (quiescent) CD8⁺ T cells of the blood, spleen and mesenteric lymph nodes were identified on the basis of surface coexpression of CD45RA and CD62L. Relative to age-matched controls, the percentage of naïve-phenotype CD8⁺ T cells was high in energy-restricted groups after 9 d and 14 d of weight loss and in the protein-restricted groups after 14 d (P 0.05). No ontogenetic change was apparent (age-matched vs. zero-time control). Other studies have demonstrated depression in cell-mediated immune competence in both malnutrition models within the first week of weight loss. An overabundance of quiescent-phenotype T cells within the involuted CD8⁺ compartment may contribute to established immune depression but not to its initiation in weight loss pathologies.

KEY WORDS: • mice • T cell • blood • spleen • lymph node

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