![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville FL 32611-0370
2To whom correspondence should be addressed. E-mail: rjc{at}gnv.ifas.ufl.edu.
Zinc metabolism is well regulated over a wide range of dietary intakes to help maintain cellular zinc-dependent functions. Expression of transporter molecules, which influence zinc influx and efflux across the plasma and intracellular membranes, contributes to this regulation. We have examined in rats the comparative response of zinc transporters 1, 2, and 4 (ZnT-1, ZnT-2 and ZnT-4) to dietary zinc. ZnT-1 and ZnT-4 are expressed ubiquitously, whereas ZnT-2 is limited to small intestine, kidney, placenta and, in some cases, the liver. When zinc intake was low (<1 mg Zn/kg), ZnT-2 mRNA was extremely low in small intestine and kidney compared with an adequate intake (30 mg Zn/kg). ZnT-1 and ZnT-2 mRNAs were markedly greater in both tissues when a supplemental zinc intake (180 mg Zn/kg) was provided. ZnT-4 was refractory to changes in zinc intake. When zinc was provided as a single oral dose (70 mg/kg body), ZnT-1 and ZnT-2 mRNA levels were increased many fold in small intestine, liver and kidney, whereas ZnT-4 gene expression was not changed. The expression of ZnT-1 and ZnT-2 is comparable to zinc-induced changes in metallothionein mRNA levels, suggesting a similar mode of regulation for these genes. The relative differential in regulation by zinc is ZnT-2 > ZnT-1 > ZnT-4. These data provide evidence that, in an animal model, zinc transporter expression is responsive to zinc under physiologically relevant conditions.
KEY WORDS: • zinc • zinc transporter • rats • gene expression • zinc metabolism
This article has been cited by other articles:
|
|
 |
|
  M.-Y. Jou, A. G. Hall, A. F. Philipps, S. L. Kelleher, and B. Lonnerdal Tissue-Specific Alterations in Zinc Transporter Expression in Intestine and Liver Reflect a Threshold for Homeostatic Compensation during Dietary Zinc Deficiency in Weanling Rats J. Nutr., May 1, 2009; 139(5): 835 - 841. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Zheng, G. P. Feeney, P. Kille, and C. Hogstrand Regulation of ZIP and ZnT zinc transporters in zebrafish gill: zinc repression of ZIP10 transcription by an intronic MRE cluster Physiol Genomics, July 1, 2008; 34(2): 205 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yu, L. Lu, X. G. Luo, and B. Liu Kinetics of Zinc Absorption by In Situ Ligated Intestinal Loops of Broilers Involved in Zinc Transporters Poult. Sci., June 1, 2008; 87(6): 1146 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Overbeck, P. Uciechowski, M. L. Ackland, D. Ford, and L. Rink Intracellular zinc homeostasis in leukocyte subsets is regulated by different expression of zinc exporters ZnT-1 to ZnT-9 J. Leukoc. Biol., February 1, 2008; 83(2): 368 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. L. Huang, L. Lu, X. G. Luo, and B. Liu An Optimal Dietary Zinc Level of Broiler Chicks Fed a Corn-Soybean Meal Diet Poult. Sci., December 1, 2007; 86(12): 2582 - 2589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Jackson, R. M. Helston, J. A. McKay, E. D. O'Neill, J. C. Mathers, and D. Ford Splice Variants of the Human Zinc Transporter ZnT5 (SLC30A5) Are Differentially Localized and Regulated by Zinc through Transcription and mRNA Stability J. Biol. Chem., April 6, 2007; 282(14): 10423 - 10431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Y. Yu, C. P. Kirschke, and L. Huang Immunohistochemical Analysis of ZnT1, 4, 5, 6, and 7 in the Mouse Gastrointestinal Tract J. Histochem. Cytochem., March 1, 2007; 55(3): 223 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Chung, C. Hogstrand, and S.-J. Lee Cytotoxicity of nitric oxide is alleviated by zinc-mediated expression of antioxidant genes. Experimental Biology and Medicine, October 1, 2006; 231(9): 1555 - 1563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Cousins, J. P. Liuzzi, and L. A. Lichten Mammalian Zinc Transport, Trafficking, and Signals J. Biol. Chem., August 25, 2006; 281(34): 24085 - 24089. [Full Text] [PDF]
|
|
|
|
 |
|
 T. B. Aydemir, R. K. Blanchard, and R. J. Cousins Zinc supplementation of young men alters metallothionein, zinc transporter, and cytokine gene expression in leukocyte populations PNAS, February 7, 2006; 103(6): 1699 - 1704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. King, J. W. Frentzel, J. J. Mann, and P. J. Fraker Chronic Zinc Deficiency in Mice Disrupted T Cell Lymphopoiesis and Erythropoiesis While B Cell Lymphopoiesis and Myelopoiesis Were Maintained J. Am. Coll. Nutr., December 1, 2005; 24(6): 494 - 502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Dufner-Beattie, Z. L. Huang, J. Geiser, W. Xu, and G. K. Andrews Generation and Characterization of Mice Lacking the Zinc Uptake Transporter ZIP3 Mol. Cell. Biol., July 1, 2005; 25(13): 5607 - 5615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Chowanadisai, S. L. Kelleher, and B. Lonnerdal Zinc Deficiency Is Associated with Increased Brain Zinc Import and LIV-1 Expression and Decreased ZnT-1 Expression in Neonatal Rats J. Nutr., May 1, 2005; 135(5): 1002 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R A Cragg, S R Phillips, J M Piper, J S Varma, F C Campbell, J C Mathers, and D Ford Homeostatic regulation of zinc transporters in the human small intestine by dietary zinc supplementation Gut, April 1, 2005; 54(4): 469 - 478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Chimienti, S. Devergnas, A. Favier, and M. Seve Identification and Cloning of a {beta}-Cell-Specific Zinc Transporter, ZnT-8, Localized Into Insulin Secretory Granules Diabetes, September 1, 2004; 53(9): 2330 - 2337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. B. Andree, J. Kim, C. P. Kirschke, J. P. Gregg, H. Paik, H. Joung, L. Woodhouse, J. C. King, and L. Huang Investigation of Lymphocyte Gene Expression for Use as Biomarkers for Zinc Status in Humans J. Nutr., July 1, 2004; 134(7): 1716 - 1723. [Abstract] [Full Text]
|
|
|
|
|
|
J. Dufner-Beattie, S. J. Langmade, F. Wang, D. Eide, and G. K. Andrews Structure, Function, and Regulation of a Subfamily of Mouse Zinc Transporter Genes J. Biol. Chem., December 12, 2003; 278(50): 50142 - 50150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L Kelleher and B. Lonnerdal Zn Transporter Levels and Localization Change Throughout Lactation in Rat Mammary Gland and Are Regulated by Zn in Mammary Cells J. Nutr., November 1, 2003; 133(11): 3378 - 3385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dufner-Beattie, F. Wang, Y.-M. Kuo, J. Gitschier, D. Eide, and G. K. Andrews The Acrodermatitis Enteropathica Gene ZIP4 Encodes a Tissue-specific, Zinc-regulated Zinc Transporter in Mice J. Biol. Chem., August 29, 2003; 278(35): 33474 - 33481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Lee, W. Woo, B. Wu, A. Kummer, H. Duminy, and Z. Xu Zinc Accumulation in N-Methyl-N-Nitrosourea-Induced Rat Mammary Tumors Is Accompanied by an Altered Expression of ZnT-1 and Metallothionein Experimental Biology and Medicine, June 1, 2003; 228(6): 689 - 696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Cousins, R. K. Blanchard, J. B. Moore, L. Cui, C. L. Green, J. P. Liuzzi, J. Cao, and J. A. Bobo Regulation of Zinc Metabolism and Genomic Outcomes J. Nutr., May 1, 2003; 133(5): 1521S - 1526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Liuzzi, J. A. Bobo, L. Cui, R. J. McMahon, and R. J. Cousins Zinc Transporters 1, 2 and 4 Are Differentially Expressed and Localized in Rats during Pregnancy and Lactation J. Nutr., February 1, 2003; 133(2): 342 - 351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Kirschke and L. Huang ZnT7, a Novel Mammalian Zinc Transporter, Accumulates Zinc in the Golgi Apparatus J. Biol. Chem., January 31, 2003; 278(6): 4096 - 4102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ranaldi, G. Perozzi, A. Truong-Tran, P. Zalewski, and C. Murgia Intracellular distribution of labile Zn(II) and zinc transporter expression in kidney and MDCK cells Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1365 - F1375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Kelleher and B. Lonnerdal Zinc Transporters in the Rat Mammary Gland Respond to Marginal Zinc and Vitamin A Intakes during Lactation J. Nutr., November 1, 2002; 132(11): 3280 - 3285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Cragg, G. R. Christie, S. R. Phillips, R. M. Russi, S. Kury, J. C. Mathers, P. M. Taylor, and D. Ford A Novel Zinc-regulated Human Zinc Transporter, hZTL1, Is Localized to the Enterocyte Apical Membrane J. Biol. Chem., June 14, 2002; 277(25): 22789 - 22797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Blanchard, J. B. Moore, C. L. Green, and R. J. Cousins Inaugural Article: Modulation of intestinal gene expression by dietary zinc status: Effectiveness of cDNA arrays for expression profiling of a single nutrient deficiency PNAS, November 20, 2001; 98(24): 13507 - 13513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cao, J. A. Bobo, J. P. Liuzzi, and R. J. Cousins Effects of intracellular zinc depletion on metallothionein and ZIP2 transporter expression and apoptosis J. Leukoc. Biol., October 1, 2001; 70(4): 559 - 566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Gaither and D. J. Eide The Human ZIP1 Transporter Mediates Zinc Uptake in Human K562 Erythroleukemia Cells J. Biol. Chem., June 15, 2001; 276(25): 22258 - 22264. [Abstract] [Full Text] [PDF]
|
|
