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Article

Food Deprivation Exacerbates Mitochondrial Oxidative Stress in Rat Liver Exposed to Ischemia-Reperfusion Injury¹

Marco Domenicali, Paolo Caraceni², Gianluigi Vendemiale, Ignazio Grattagliano, Bruno Nardo^*, Monia Dall’Agata, Bruno Santoni^*, Franco Trevisani, Antonino Cavallari^*, Emanuele Altomare and Mauro Bernardi

Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia e ^* Dipartimento di Discipline Chirurgiche, Rianimatorie e dei Trapianti, University of Bologna, 40138 Bologna, Italy and Dipartimento di Medicina Interna e Pubblica University of Bari, 70124 Bari, Italy

²To whom correspondence should be addressed. E-mail: caraceni{at}med.unibo.it.

Mitochondria undergo oxidative damage during reperfusion of ischemic liver. Although nutritional status affects ischemia-reperfusion injury in the liver, its effect on mitochondrial damage has not been evaluated. Thus, this study was designed to determine whether starvation influences the oxidative balance in mitochondria isolated from livers exposed to warm ischemia-reperfusion. Fed and 18- and 36-h food-deprived rats underwent partial hepatic ischemia followed by reperfusion. Mitochondria were isolated before and after ischemia and during reperfusion. Serum alanine transaminase was measured to assess liver injury. The mitochondrial concentrations of malondialdehyde, protein carbonyls and glutathione were determined as indicators of oxidative injury. Cell ultrastructure was assessed by transmission electron microscopy. Transaminase levels were greater in 18-h food-deprived than fed rats (after 120 min of reperfusion: 3872 ± 400 vs. 1138 ± 59 U/L, P < 0.01). Mitochondrial glutathione was lower in food-deprived than fed rats before and after ischemia, and during reperfusion. Food deprivation also was associated with significantly greater lipid and protein oxidative damage. Finally, more ultrastructural damage was observed during reperfusion in mitochondria from food-deprived rats. Prolonging the length of food deprivation to 36 h exacerbated significantly both the mitochondrial oxidative injury and the release of serum transaminases in rats (after 120 min of reperfusion: 5438 ± 504 U/L, P < 0.01). Food deprivation was associated with greater mitochondrial oxidative injury in rat livers exposed to warm ischemia-reperfusion, and the extent of oxidative damage in mitochondria increased with the length of food deprivation.

KEY WORDS: • food deprivation • mitochondria • ischemia-reperfusion injury • oxidative stress • rats

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