QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bezerra, R. M. N. Articles by Saad, M. J. A. Search for Related Content PubMed PubMed Citation Articles by Bezerra, R. M. N. Articles by Saad, M. J. A.

---

Article

A High Fructose Diet Affects the Early Steps of Insulin Action in Muscle and Liver of Rats¹

Departamento de Planejamento Alimentar e Nutrição, Faculdade de Engenharia de Alimentos and ^* Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brasil.

²To whom correspondence should be addressed.

A high fructose diet induces insulin resistance in rats, although the exact molecular mechanism involved is unknown. In this study, we used immunoprecipitation and immunoblotting to examine the levels and phosphorylation status of the insulin receptor (IR) and insulin receptor substrate-1 (IRS-1), as well as the association of the IRS-1 with phosphatidylinositol 3-kinase (PI 3-kinase), and phosphotyrosine phosphatase (SHP2) in the liver and muscle of rats fed a control or high fructose diet for 28 d. There were no differences in IR and the IRS-1 protein levels in the liver and muscle of rats fed the control and high fructose diets. However, tyrosine-phosphorylation of the insulin receptor after insulin stimulation was reduced to 71 ± 2% (P < 0.05) of control in the liver of the fructose-fed rats. In samples previously immunoprecipitated with anti-IRS-1 antibody and blotted with antiphosphotyrosine antibody, the insulin-stimulated IRS-1 phosphorylation levels in the liver and muscle of the fructose-fed group were only 70 ± 6% (P < 0.05) and 76 ± 5% (P < 0.05) of those of control rats, respectively. The insulin-stimulated IRS-1 association with PI 3-kinase was reduced to 84 ± 3% (P < 0.05) in the liver and to 84 ± 4% (P < 0.05) in the muscle of the fructose-fed group compared with control rats. Insulin-stimulated IRS-1 association with SHP2 was reduced to 79 ± 5% (P < 0.05) in liver of the fructose-fed rats. These data suggest that changes in the early steps of insulin signal transduction may have an important role in the insulin resistance observed in these rats.

KEY WORDS: • fructose • insulin receptor • insulin receptor substrate-1 • phosphatidylinositol 3-kinase • phosphotyrosine phosphatase • rats

This article has been cited by other articles:

				L. M. Federico, M. Naples, D. Taylor, and K. Adeli Intestinal Insulin Resistance and Aberrant Production of Apolipoprotein B48 Lipoproteins in an Animal Model of Insulin Resistance and Metabolic Dyslipidemia: Evidence for Activation of Protein Tyrosine Phosphatase-1B, Extracellular Signal-Related Kinase, and Sterol Regulatory Element-Binding Protein-1c in the Fructose-Fed Hamster Intestine. Diabetes, May 1, 2006; 55(5): 1316 - 1326. [Abstract] [Full Text] [PDF]

				Y. Wei, D. Wang, and M. J. Pagliassotti Fructose Selectively Modulates c-jun N-Terminal Kinase Activity and Insulin Signaling in Rat Primary Hepatocytes J. Nutr., July 1, 2005; 135(7): 1642 - 1646. [Abstract] [Full Text] [PDF]

				J. M. Zabolotny, F. G. Haj, Y.-B. Kim, H.-J. Kim, G. I. Shulman, J. K. Kim, B. G. Neel, and B. B. Kahn Transgenic Overexpression of Protein-tyrosine Phosphatase 1B in Muscle Causes Insulin Resistance, but Overexpression with Leukocyte Antigen-related Phosphatase Does Not Additively Impair Insulin Action J. Biol. Chem., June 4, 2004; 279(23): 24844 - 24851. [Abstract] [Full Text] [PDF]

				Y. Wei, M. E. Bizeau, and M. J. Pagliassotti An Acute Increase in Fructose Concentration Increases Hepatic Glucose-6-Phosphatase mRNA via Mechanisms That Are Independent of Glycogen Synthase Kinase-3 in Rats J. Nutr., March 1, 2004; 134(3): 545 - 551. [Abstract] [Full Text] [PDF]

				Y. Nishimoto, T. Tomida, H. Matsui, T. Ito, and K. Okumura Decrease in Renal Medullary Endothelial Nitric Oxide Synthase of Fructose-Fed, Salt-Sensitive Hypertensive Rats Hypertension, August 1, 2002; 40(2): 190 - 194. [Abstract] [Full Text] [PDF]

				V. A. ZAMMIT Insulin Stimulation of Hepatic Triacylglycerol Secretion in the Insulin-Replete State: Implications for the Etiology of Peripheral Insulin Resistance Ann. N.Y. Acad. Sci., June 1, 2002; 967(1): 52 - 65. [Abstract] [Full Text] [PDF]

				G. J. COONEY, A. L. THOMPSON, S. M. FURLER, J. YE, and E. W. KRAEGEN Muscle Long-Chain Acyl CoA Esters and Insulin Resistance Ann. N.Y. Acad. Sci., June 1, 2002; 967(1): 196 - 207. [Abstract] [Full Text] [PDF]

				C. Taghibiglou, F. Rashid-Kolvear, S. C. Van Iderstine, H. Le-Tien, I. G. Fantus, G. F. Lewis, and K. Adeli Hepatic Very Low Density Lipoprotein-ApoB Overproduction Is Associated with Attenuated Hepatic Insulin Signaling and Overexpression of Protein-tyrosine Phosphatase 1B in a Fructose-fed Hamster Model of Insulin Resistance J. Biol. Chem., January 4, 2002; 277(1): 793 - 803. [Abstract] [Full Text]

				M. J. Pagliassotti, J. Kang, J. S. Thresher, C. K. Sung, and M. E. Bizeau Elevated basal PI 3-kinase activity and reduced insulin signaling in sucrose-induced hepatic insulin resistance Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E170 - E176. [Abstract] [Full Text] [PDF]