QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lingelbach, L. B. Articles by McDonald, R. B. Search for Related Content PubMed PubMed Citation Articles by Lingelbach, L. B. Articles by McDonald, R. B.

---

Article

Accumulation of Advanced Glycation Endproducts in Aging Male Fischer 344 Rats during Long-Term Feeding of Various Dietary Carbohydrates¹

Department of Nutrition, University of California, Davis, CA 95616-8669

²To whom correspondence should be addressed.

The observation of accelerated collagen glycation in association with enhanced progression of many age-associated diseases in hyperglycemic subjects has led researchers to propose a role of glycation in the aging process. Although short-term studies in healthy animals suggest that feeding a diet high in fructose may increase serum glucose concentrations and increase glycemic stress, the effects of a long-term feeding, i.e., life span, are unknown. This study was designed to evaluate the long-term effects of dietary carbohydrates on serum and tissue markers of glycemic stress. Three-month-old male Fischer 344 rats were given free access to or restricted to 60% caloric intake of one of five isocaloric diets that contained as their carbohydrate source either cornstarch, glucose, sucrose, fructose or equimolar amounts of fructose and glucose. Rats were killed at 9-, 18- or 26-mo of age. Glycated hemoglobin, serum glucose and fructosamine levels were measured as markers of serum glycemic stress. Collagen-associated fluorescence and pentosidine concentrations were measured in skin, aortic, tracheal and tail tendon collagen as markers of advanced glycation endproducts (AGE). The source of dietary carbohydrate had little effect on markers of glycemic stress and the accumulation of AGE. Restricting the amount of calories consumed resulted in lower serum glucose concentrations, glycated hemoglobin levels and pentosidine concentrations in tail tendon collagen. Our data suggest that the rate of collagen glycation is tissue-specific. These results suggest that long-term feeding of specific dietary carbohydrates does not alter serum glucose concentrations or the rate of collagen glycation. Rather, age-related accumulation of AGE is more closely related to caloric intake.

KEY WORDS: • collagen crosslinks • collagen-associated fluorescence • energy restriction • pentosidine • Fischer F344 rats

This article has been cited by other articles:

				B. Bartling, M. Desole, S. Rohrbach, R.-E. Silber, and A. Simm Age-associated changes of extracellular matrix collagen impair lung cancer cell migration FASEB J, May 1, 2009; 23(5): 1510 - 1520. [Abstract] [Full Text] [PDF]

				L. B. Lingelbach and R. B. McDonald Description of the Long-Term Lipogenic Effects of Dietary Carbohydrates in Male Fischer 344 Rats J. Nutr., December 1, 2000; 130(12): 3077 - 3084. [Abstract] [Full Text] [PDF]