QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Andlauer, W. Articles by Fürst, P. Search for Related Content PubMed PubMed Citation Articles by Andlauer, W. Articles by Fürst, P.

---

Article

Absorption and Metabolism of Genistein in Isolated Rat Small Intestine

Wilfried Andlauer^*1, Jochen Kolb^*, Peter Stehle and Peter Fürst^*

^* Institute for Biological Chemistry and Nutrition, University of Hohenheim, D-70593 Stuttgart, Germany; and Department of Nutrition Science, University of Bonn, D-53115 Bonn, Germany

¹To whom correspondence should be addressed.

Studies suggest a variety of biological effects for the isoflavonoid genistein, but there is little information regarding small intestinal absorption and metabolism. The aim of this study was to investigate the absorption and metabolism of luminally administered genistein in an isolated preparation of luminally and vascularly perfused rat small intestine. A synthetic perfusate free from blood components was used as vascular medium, with a perfluorocarbon as oxygen carrier. Luminal media consisted of a bicarbonate buffered sodium chloride solution spiked with genistein (12 µmol/L). Viability was maintained during the entire perfusion as indicated by no significant differences between genistein and control perfusions for perfusion pressure, lactate-pyruvate ratio, oxygen uptake and acid-base homeostasis. Luminal disappearance rate of genistein did not change throughout the entire perfusion time. After a significant increase until about 30 min, vascular appearance rate of total genistein reached an equilibrium. The intestinal absorption of luminally administered genistein was 40.6%, corresponding to an average uptake of 2.9 nmol · min^-1 · g dry intestine^-1. The majority (31.3%) of the absorbed genistein appeared as genistein glucuronide, also recovered as the main metabolite on the luminal side (13.3%). Only small amounts of genistein (2.6%) and genistein glucuronide (2.9%) were found in the intestinal tissue. The results demonstrate a favorable uptake of genistein, a pertinent addition to the ongoing discussion about health benefits of isoflavones.

KEY WORDS: • intestinal absorption • genistein • isoflavone • intestinal metabolism • rats

This article has been cited by other articles:

				K. R Walsh, S. J Haak, T. Bohn, Q. Tian, S. J Schwartz, and M. L Failla Isoflavonoid glucosides are deconjugated and absorbed in the small intestine of human subjects with ileostomies Am. J. Clinical Nutrition, April 1, 2007; 85(4): 1050 - 1056. [Abstract] [Full Text] [PDF]

				A.-C. Chen and S. M. Donovan Genistein at a Concentration Present in Soy Infant Formula Inhibits Caco-2BBe Cell Proliferation by Causing G2/M Cell Cycle Arrest J. Nutr., June 1, 2004; 134(6): 1303 - 1308. [Abstract] [Full Text] [PDF]

				W. A. Fritz, M. S. Cotroneo, J. Wang, I.-E. Eltoum, and C. A. Lamartiniere Dietary Diethylstilbestrol but Not Genistein Adversely Affects Rat Testicular Development J. Nutr., July 1, 2003; 133(7): 2287 - 2293. [Abstract] [Full Text] [PDF]

				K. D. R. Setchell, N. M. Brown, and E. Lydeking-Olsen The Clinical Importance of the Metabolite Equol--A Clue to the Effectiveness of Soy and Its Isoflavones J. Nutr., December 1, 2002; 132(12): 3577 - 3584. [Abstract] [Full Text] [PDF]

				K. D. Setchell, N. M Brown, L. Zimmer-Nechemias, W. T Brashear, B. E Wolfe, A. S Kirschner, and J. E Heubi Evidence for lack of absorption of soy isoflavone glycosides in humans, supporting the crucial role of intestinal metabolism for bioavailability Am. J. Clinical Nutrition, August 1, 2002; 76(2): 447 - 453. [Abstract] [Full Text] [PDF]

				K. Murota, S. Shimizu, S. Miyamoto, T. Izumi, A. Obata, M. Kikuchi, and J. Terao Unique Uptake and Transport of Isoflavone Aglycones by Human Intestinal Caco-2 Cells: Comparison of Isoflavonoids and Flavonoids J. Nutr., July 1, 2002; 132(7): 1956 - 1961. [Abstract] [Full Text] [PDF]

				A. Adam, V. Crespy, M.-A. Levrat-Verny, F. Leenhardt, M. Leuillet, C. Demigne, and C. Remesy The Bioavailability of Ferulic Acid Is Governed Primarily by the Food Matrix Rather than Its Metabolism in Intestine and Liver in Rats J. Nutr., July 1, 2002; 132(7): 1962 - 1968. [Abstract] [Full Text] [PDF]

				S. Lyou, E. Hirano, K. Tujioka, Y. Mawatari, K. Hayase, S. Okuyama, and H. Yokogoshi Dietary Genistein Affects Brain Protein Synthesis Rates in Ovariectomized Female Rats J. Nutr., July 1, 2002; 132(7): 2055 - 2058. [Abstract] [Full Text] [PDF]

				W. Andlauer, J. Kolb, and P. Furst Isoflavones from Tofu Are Absorbed and Metabolized in the Isolated Rat Small Intestine J. Nutr., December 1, 2000; 130(12): 3021 - 3027. [Abstract] [Full Text] [PDF]