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Article

Zn-Depleted Mice Absorb More of an Intragastric Zn Solution by a Metallothionein-Enhanced Process Than Do Zn-Replete Mice

Division of Clinical Biochemistry, Institute of Medical and Veterinary Science, Adelaide, SA 5000 Australia

¹To whom correspondence should be addressed.

The influence of metallothionein (MT)² on Zn absorption was investigated in MT-null (MT-/-) and normal (MT+/+) mice fed Zn-depleted (ZnD) diets for 7 d and compared with those fed Zn-replete (ZnR) diets in a previous study. Mice were starved for 20 h, then administered an oral gavage of aqueous ⁶⁵ZnSO₄ solution at doses of 154, 770 or 1540 nmol of Zn, and the amount transferred into nongut tissues was determined 4 h later. ⁶⁵Zn transfer did not differ between genotypes in ZnR mice. However ZnD MT+/+ mice had a 30–40% greater transfer from the 154 and 770 Zn doses compared to ZnR MT+/+ mice. This was not observed in MT-/- mice. In MT+/+ mice, Zn depletion enhanced the induction of MT by Zn in the intestine and pancreas. ⁶⁵Zn uptakes in the liver and pancreas were greater in MT+/+ than MT-/- mice, and this was greater (50%) at the 154 and 770 doses in mice fed ZnD diets. Plasma Zn concentrations were raised to a similar extent in ZnR and ZnD MT-/- mice. ZnR MT+/+ mice had significantly lower plasma Zn levels than MT-/-mice; this difference was less marked in the ZnD mice. We conclude that a MT-facilitated enhancement in Zn absorption occurs in response to dietary Zn deficiency.

KEY WORDS: • Zinc • metallothionein • intestinal absorption • metallothionein-null mice • Zn deficiency

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