![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
*
INRA, Unité de Recherches sur les Herbivores, Theix, 63122 Saint-Genès-Champanelle, France; and
INRA, Laboratoire de Génétique biochimique et de Cytogénétique, 78352 Jouy-en-Josas cedex, France
4To whom correspondence should be addressed.
Previous studies in rodents have shown that the lipoprotein lipase (LPL) regulation is complex and often opposite in adipose tissue (AT) and muscle in response to the same nutritional treatment. However, neither LPL responses nor the molecular mechanisms involved in the nutritional regulation have been studied in both AT and muscle of ruminant species. To explore this, we measured the LPL activity and mRNA levels in perirenal AT and cardiac muscle (CM) of control, 7-d-underfed or 14-d-refed ewes. Underfeeding decreased (P < 0.01) LPL activity both in AT (-59%) and CM (-31%), and these activities were restored (P < 0.01) by refeeding (AT, +248%; CM, +34%). Variations of LPL mRNA level measured by real-time reverse transcription-polymerase chain reaction or by Northern blot followed variations of LPL activity: underfeeding decreased AT- and CM-LPL mRNA levels (-58 and -53%, respectively), and refeeding restored (P < 0.01) them in CM (+117%) and increased them over the baseline in AT (+640%). Quantification of either 3.4- or 3.8-kb LPL mRNA levels revealed a predominant (P < 0.001) expression of the 3.4-kb mRNA in AT (60%) and of the 3.8-kb mRNA in CM (56%), without any preferential regulation of one of these mRNA species by the nutritional status. This work reveals a tissue-specific expression pattern of the ovine LPL gene and a pretranslational nutritional regulation of its expression, which is achieved in the same direction in perirenal AT and CM. The different regulation of CM-LPL between ewes and rats probably arises from peculiarities of ruminant species for nutrient digestion and absorption and liver lipogenesis.
KEY WORDS: • LPL gene expression • nutritional status • adipose tissue • cardiac muscle • sheep
This article has been cited by other articles:
|
|
 |
|
  M. Bonnet, Y. Faulconnier, C. Leroux, C. Jurie, I. Cassar-Malek, D. Bauchart, P. Boulesteix, D. Pethick, J. F. Hocquette, and Y. Chilliard Glucose-6-phosphate dehydrogenase and leptin are related to marbling differences among Limousin and Angus or Japanese Black x Angus steers J Anim Sci, November 1, 2007; 85(11): 2882 - 2894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jurie, I. Cassar-Malek, M. Bonnet, C. Leroux, D. Bauchart, P. Boulesteix, D. W. Pethick, and J. F. Hocquette Adipocyte fatty acid-binding protein and mitochondrial enzyme activities in muscles as relevant indicators of marbling in cattle J Anim Sci, October 1, 2007; 85(10): 2660 - 2669. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ollier, C. Robert-Granie, L. Bernard, Y. Chilliard, and C. Leroux Mammary Transcriptome Analysis of Food-Deprived Lactating Goats Highlights Genes Involved in Milk Secretion and Programmed Cell Death J. Nutr., March 1, 2007; 137(3): 560 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Bernard, J. Rouel, C. Leroux, A. Ferlay, Y. Faulconnier, P. Legrand, and Y. Chilliard Mammary Lipid Metabolism and Milk Fatty Acid Secretion in Alpine Goats Fed Vegetable Lipids J Dairy Sci, April 1, 2005; 88(4): 1478 - 1489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Lee and K. L. Hossner Coordinate regulation of ovine adipose tissue gene expression by propionate J Anim Sci, November 1, 2002; 80(11): 2840 - 2849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Eckhardt Genetic Research and Nutritional Individuality J. Nutr., February 1, 2001; 131(2): 336S - 339. [Abstract] [Full Text]
|
|
