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Research Communication

Hepatic Ascorbic Acid Saturation Is the Most Stringent Response Criterion for Determining the Vitamin C Requirement of Juvenile European Sea Bass (Dicentrarchus labrax)

Vincent Fournier^*, Marie F. Gouillou-Coustans^*1 and Sadasivam J. Kaushik

Fish Nutrition Laboratory, Unité mixte INRA-IFREMER, ^* B.P 70, 29280 Plouzané, Station d’Hydrobiologie, INRA 64310 St. Pée-sur-Nivelle, France

¹To whom correspondence should be addressed.

Our main objective was to verify whether the dietary ascorbic acid (AA) requirement of juvenile European sea bass (Dicentrarchus labrax) varies as a function of different physiological needs. Practical diets with eight (0, 5, 10, 20, 40, 80, 160, 320 mg AA/kg diet) levels of ascorbic acid polyphosphate were fed to sea bass (mean weight: 0.7 g) for 15 wk. At the beginning and at the end of the feeding trial, tissues were sampled for vitamin C and hydroxyproline (HyPro) analysis. Dose-dependent responses of skin and whole body HyPro concentrations and hepatic AA concentration to dietary vitamin C levels were observed. Skin and whole body HyPro concentrations were low in sea bass fed AA-deficient diet, 217 and 15 nmol/g tissue, respectively. HyPro levels increased with increasing dietary levels, reaching plateaus of 297 and 45 nmol/g tissue in the skin and whole body at dietary vitamin C levels of at least 5 and 31 mg AA/kg. Hepatic AA level increased with increasing dietary levels, reaching a plateau of 474 pmol/g tissue in juveniles fed at least 121 mg of AA/kg. We concluded that hepatic AA saturation is the most stringent response criterion for determination of the vitamin C requirement in juvenile European sea bass.

KEY WORDS: • ascorbic acid • requirement • hepatic saturation • hydroxyproline • Dicentrarchus labrax

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