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Articles

Glucose Is Absorbed in a Sodium-Dependent Manner from Forestomach Contents of Sheep

Department of Veterinary Physiology, Leipzig University, D-04103 Leipzig, Germany and ^* Department of Animal Nutrition, CCS Haryana Agricultural University, Hisar-125004, India

³To whom correspondence should be addressed.

Intraruminal glucose is thought to be completely converted to short-chain fatty acids (SCFA) by symbiotic microorganisms. Nevertheless, earlier in vitro studies evidenced the expression of the sodium glucose-linked transporter (SGLT)-1, in the ovine ruminal epithelium. The present study aimed to determine whether the ruminal SGLT-1 is functionally important in vivo. In a first experimental series using the emptied, washed, and isolated reticulorumen of sheep, 6.3% of glucose was absorbed from an intraruminal buffer solution (2 L, 128 mmol/L Na⁺, 0.5 mmol/L glucose, 0 mmol/L galactose) within 30 min (P < 0.001). Reducing Na⁺ concentration to 10 mmol/L resulted in complete inhibition of glucose absorption, and the addition of 10 mmol/L galactose (at 128 mmol/L Na⁺) induced a small but insignificant inhibition. In a second experimental series, the addition of 12 mmol/L glucose to an initially glucose-free buffer led to an increase in the transruminal potential difference from 34.4 to 37.1 mV within 4 min (P < 0.001). From the 12 mmol/L glucose-containing buffer, 11.0% of glucose was absorbed within 30 min (P < 0.05). In all experiments, microbial glucose degradation in the reticulorumen was prevented by adding cefuroxime (100 mg/L) and colistin methanesulfonate (25 mg/L) to the buffer solution. The effectiveness of antimicrobial treatment was verified by ex vivo incubations of buffer samples drawn from the reticulorumen. We conclude that glucose is absorbed in a sodium-dependent manner from the reticulorumen at low and high glucose concentrations. Absorption at high glucose concentrations is of nutritional importance because it counteracts the genesis of ruminal lactic acidosis.

KEY WORDS: • ruminants • sheep • rumen • carbohydrates • sodium glucose-linked transport (SGLT)

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				J. R. Aschenbach, T. Borau, and G. Gabel Glucose Uptake via SGLT-1 Is Stimulated by {beta}2-Adrenoceptors in the Ruminal Epithelium of Sheep J. Nutr., June 1, 2002; 132(6): 1254 - 1257. [Abstract] [Full Text] [PDF]