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2
*
Parke-Davis Laboratory for Molecular Genetics, Alameda, CA 96501 and
Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA 70808
2To whom correspondence should be addressed.
AKR/J mice fed a high fat diet were treated with a 1% (1 g/100 g)
admixture of conjugated linoleic acids (CLA) for 5 wk and compared with
control mice. Body weights, energy intakes and energy expenditure (EE)
determined by indirect calorimetry were measured weekly. CLA treatment
reduced adipose depot weights by 
50% but had no significant effects
on either body weight or energy intake. CLA increased EE persistently
by an average of 7.7% throughout the 5-wk experiment. This greater EE,
despite no difference in energy intake, was sufficient to account for
the lower body fat stores in the CLA-treated mice. De novo fatty
acid biosynthesis in adipose tissue, measured by incorporation of
deuterium-labeled water, was not decreased by CLA treatment and
therefore did not explain the lower adipose lipid in these mice.
Expression of uncoupling protein (UCP) in skeletal muscle, white
adipose tissue and kidney was not affected by CLA treatment. In brown
adipose tissue, UCP1 expression was not affected by CLA treatment.
However, UCP2 expression, although quite low, was significantly greater
in CLA-fed mice. We conclude that CLA acts to reduce body fat
stores by chronically increasing metabolic rate. This effect on
metabolic rate is likely not due to increased UCP gene expression.
Furthermore, the reduced body fat is not due to decreased de novo fatty
acid synthesis in white adipose tissue.
KEY WORDS: • mice • adipose tissue • metabolic rate • uncoupling protein • growth • obesity treatment
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