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Research Communication

Kinetics of Carnitine Palmitoyltransferase-I Are Altered by Dietary Variables and Suggest a Metabolic Need for Supplemental Carnitine in Young Pigs^{1 ,2}

Department of Animal Science, North Carolina State University, Raleigh, NC 27695-7621 and ^* Department of Animal Science & Technology, Seoul National University, Suweon, Korea 441–744

³To whom correspondence should be addressed.

To examine the kinetics of carnitine palmitoyltransferase-I (CPT-I) and the influence of dietary variables, young pigs (18 kg, n = 20) were fed corn-soybean meal diets supplemented with 40 g soy oil/kg and containing either 136 or 180 g crude protein/kg and either 0 or 500 mg/kg L-carnitine (2 x 2 factorial design). Diets were offered for 10 d (85% of ad libitum); CPT-I activities in liver and skeletal muscle mitochondria were determined, and enzyme kinetic constants (V_max and K_m for carnitine) were estimated. Kinetics of CPT-I in muscle were not affected by diet (P > 0.1; carnitine K_m = 480 ± 44 µmol/L). In contrast, the K_m for carnitine in liver was increased from 164 to 216 ± 20 µmol/L by dietary L-carnitine supplementation (P < 0.01) and from 169 to 211 ± 20 µmol/L by high protein feeding (P < 0.05). Dietary L-carnitine increased muscle and liver free carnitine concentrations by 72 and 158% over control concentrations (770 and 80 µmol/kg wet muscle and liver, respectively). Because tissue carnitine concentrations were within the range of the respective K_m for both liver and muscle tissue, it is inferred that alteration of tissue carnitine concentrations via dietary supplementation could modulate CPT-I activity in young pigs.

KEY WORDS: • pigs • carnitine • protein • carnitine palmitoyltransferase-I • liver • muscle

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