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Research Communication

Plasma Diamine Oxidase Activity Is Greater in Copper-Adequate than Copper-Marginal or Copper-Deficient Rats¹

Northern Ireland Centre for Diet and Health, University of Ulster at Coleraine, BT52 ISA, Northern Ireland

²To whom correspondence should be addressed.

The object of this study was to determine whether serum diamine oxidase activity could distinguish among adequate, marginal and deficient copper status in rats. Male weanling Sprague-Dawley rats (n = 21) were randomly assigned to one of three dietary regimens, with copper concentrations of 0.52, 1.73 and 6.7 mg/kg diet. On completion of the study, body weights were significantly different among dietary groups, with copper-marginal rats displaying the highest mean weight and copper-deficient rats the lowest. Copper-deficient rats ate significantly less food than the other two groups. Rats fed the three diets had significantly different liver copper concentrations. Liver and heart superoxide dismutase and cytochrome c oxidase activities, and plasma ceruloplasmin and erythrocyte superoxide dismutase activities were significantly lower in the copper-deficient rats than in the other two groups. Plasma diamine oxidase activity was lower in both copper-deficient (0.18 ± 0.11 U/L) and marginal (0.21 ± 0.11 U/L) rats compared with copper-adequate rats (3.35 ± 0.28 U/L). Of the biochemical indices measured, only liver copper concentration (-20%) and plasma diamine oxidase activity (-94%) differed between rats fed copper-marginal and copper-adequate diets. Plasma diamine oxidase activity, therefore, may be a sensitive functional biomarker of suboptimal copper status.

KEY WORDS: • diamine oxidase • copper status • cuproenzymes • rats

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