QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rosales, F. J. Articles by Ross, A. C. Search for Related Content PubMed PubMed Citation Articles by Rosales, F. J. Articles by Ross, A. C.

---

Nutrient Interactions and Toxicity

Iron Deficiency in Young Rats Alters the Distribution of Vitamin A between Plasma and Liver and between Hepatic Retinol and Retinyl Esters

Francisco J. Rosales^,1, Jing-Tsz Jang^*, Domingo J. Piñero^*, Keith M. Erikson^*, John L. Beard^* and A. Catharine Ross

^* The Nutrition Department and The Department of Veterinary Science, Pennsylvania State University, University Park, PA 16802

¹To whom correspondence and reprint requests should be addressed.

We assessed whether iron deficiency alters the concentration of vitamin A (VA) in plasma or liver and the chemical distribution between hepatic unesterified and esterified retinol. Weanling male Sprague-Dawley rats (n = 10/group) were allocated to one of four diet groups: low iron (ID3, 3 mg of elemental iron/kg diet), marginal iron (ID15, 15 mg/kg), control diet food-restricted to the ID3 group (FR, 35 mg/kg), and control diet ad libitum consumption (AD, 35 mg/kg). Both ID3 and FR rats grew less than AD and ID15 rats. At the end of 5.5 wk, plasma retinol concentrations of the ID3 and FR rats were reduced >40% compared to ID15 and AD rats [Kruskal-Wallis test (K-W), P < 0.0042)]. Paradoxically, the hepatic VA concentration was greater in FR rats, with accumulation of more retinyl esters and retinol compared to the other dietary groups. Concentrations of hepatic retinyl esters and retinol did not differ among the other groups, but the molar ratio of hepatic retinyl esters to retinol was greater in ID3 rats (20.1 ± 1.4) compared to ID15 rats (13.8 ± 1.6, P = 0.02), AD (11.3 ± 2.1, P < 0.0042) and FR (9.5 ± 1.1, P < 0.0042). Iron deficiency may cause changes in liver and plasma VA that are refractory to VA intake, and thus a benefit may be derived from combining iron and VA supplements during nutrition interventions.

KEY WORDS: • hemoglobin • hepatic retinyl esters • linear regression • plasma retinol • rat

This article has been cited by other articles:

				S.-i. Katsumata, R. Katsumata-Tsuboi, M. Uehara, and K. Suzuki Severe Iron Deficiency Decreases Both Bone Formation and Bone Resorption in Rats J. Nutr., February 1, 2009; 139(2): 238 - 243. [Abstract] [Full Text] [PDF]

				J. M. Fernandez-Real, J. M. Moreno, and W. Ricart Circulating Retinol-Binding Protein-4 Concentration Might Reflect Insulin Resistance-Associated Iron Overload Diabetes, July 1, 2008; 57(7): 1918 - 1925. [Abstract] [Full Text] [PDF]

				S. E. Schroeder, M. B. Reddy, and K. L. Schalinske Retinoic Acid Modulates Hepatic Iron Homeostasis in Rats by Attenuating the RNA-Binding Activity of Iron Regulatory Proteins J. Nutr., December 1, 2007; 137(12): 2686 - 2690. [Abstract] [Full Text] [PDF]

				J. M Graham, M. J Haskell, P. Pandey, R. K Shrestha, K. H Brown, and L. H Allen Supplementation with iron and riboflavin enhances dark adaptation response to vitamin A-fortified rice in iron-deficient, pregnant, nightblind Nepali women Am. J. Clinical Nutrition, May 1, 2007; 85(5): 1375 - 1384. [Abstract] [Full Text] [PDF]

				S. A. Tanumihardjo Assessing Vitamin A Status: Past, Present and Future J. Nutr., January 1, 2004; 134(1): 290S - 293. [Abstract] [Full Text] [PDF]

				F. T Wieringa, M. A Dijkhuizen, C. E West, D. I Thurnham, Muhilal, and J. W. Van der Meer Redistribution of vitamin A after iron supplementation in Indonesian infants Am. J. Clinical Nutrition, March 1, 2003; 77(3): 651 - 657. [Abstract] [Full Text] [PDF]

				Y. N. J. Strube, J. L. Beard, and A. C. Ross Iron Deficiency and Marginal Vitamin A Deficiency Affect Growth, Hematological Indices and the Regulation of Iron Metabolism Genes in Rats J. Nutr., December 1, 2002; 132(12): 3607 - 3615. [Abstract] [Full Text] [PDF]

				S. A. Tanumihardjo Vitamin A and Iron Status Are Improved by Vitamin A and Iron Supplementation in Pregnant Indonesian Women J. Nutr., July 1, 2002; 132(7): 1909 - 1912. [Abstract] [Full Text] [PDF]

				L. H. Allen Iron Supplements: Scientific Issues Concerning Efficacy and Implications for Research and Programs J. Nutr., April 1, 2002; 132(4): 813S - 819. [Abstract] [Full Text] [PDF]

				N. Ahluwalia, M. A. Gordon, G. Handte, M. Mahlon, N.-Q. Li, J. L. Beard, D. Weinstock, and A. C. Ross Iron Status and Stores Decline with Age in Lewis Rats J. Nutr., September 1, 2000; 130(9): 2378 - 2383. [Abstract] [Full Text]

				J.-T. Jang, J. B. Green, J. L. Beard, and M. H. Green Kinetic Analysis Shows That Iron Deficiency Decreases Liver Vitamin A Mobilization in Rats J. Nutr., May 1, 2000; 130(5): 1291 - 1296. [Abstract] [Full Text]